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Cell-Penetrating Peptides

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Cover of 'Cell-Penetrating Peptides'

Table of Contents

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    Book Overview
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    Chapter 1 Classes of Cell-Penetrating Peptides.
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    Chapter 2 Cell-Penetrating Peptides
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    Chapter 3 Prediction of Cell-Penetrating Peptides
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    Chapter 4 Computer-Aided Virtual Screening and Designing of Cell-Penetrating Peptides.
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    Chapter 5 Investigating Membrane Interactions and Structures of CPPs.
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    Chapter 6 Determining the Effects of Membrane-Interacting Peptides on Membrane Integrity.
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    Chapter 7 Study of CPP Mechanisms by Mass Spectrometry.
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    Chapter 8 Methods to Study the Role of the Glycocalyx in the Uptake of Cell-Penetrating Peptides
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    Chapter 9 Toxicity, Immunogenicity, Uptake, and Kinetics Methods for CPPs
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    Chapter 10 Unraveling the Mechanisms of Peptide-Mediated Delivery of Nucleic Acids Using Electron Microscopy.
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    Chapter 11 SCARA Involvement in the Uptake of Nanoparticles Formed by Cell-Penetrating Peptides
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    Chapter 12 Protein Mimicry and the Design of Bioactive Cell-Penetrating Peptides.
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    Chapter 13 Pepducins and Other Lipidated Peptides as Mechanistic Probes and Therapeutics.
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    Chapter 14 Identification and Characterization of Homing Peptides Using In Vivo Peptide Phage Display.
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    Chapter 15 The Antimicrobial and Antiviral Applications of Cell-Penetrating Peptides
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    Chapter 16 Visualizing Actin Architectures in Cells Incubated with Cell-Penetrating Peptides.
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    Chapter 17 Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery In Vitro.
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    Chapter 18 A Pathway Toward Tumor Cell-Selective CPPs?
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    Chapter 19 PepFects and NickFects for the Intracellular Delivery of Nucleic Acids
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    Chapter 20 In Vitro Assays to Assess Exon Skipping in Duchenne Muscular Dystrophy.
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    Chapter 21 Applications of ApoB LDLR-Binding Domain Approach for the Development of CNS-Penetrating Peptides for Alzheimer's Disease.
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    Chapter 22 CPP-Based Delivery System for In Vivo Gene Delivery
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    Chapter 23 Application of CPPs for Brain Delivery
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    Chapter 24 Intracellular Delivery of Nanoparticles with Cell Penetrating Peptides.
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    Chapter 25 Cell-Penetrating Peptides
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    Chapter 26 Cell Penetrating Peptides for Chemical Biological Studies.
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    Chapter 27 Experiences with CPP-Based Self Assembling Peptide Systems for Topical Delivery of Botulinum Toxin.
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    Chapter 28 Applications of CPPs in Genome Modulation of Plants.
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    Chapter 29 DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent.
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    Chapter 30 Live Cell Genomics: Cell-Specific Transcriptome Capture in Live Tissues and Cells.
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    Chapter 31 Live Cell Genomics: RNA Exon-Specific RNA-Binding Protein Isolation.
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    Chapter 32 ERRATUM TO: Methods to Study the Role of the Glycocalyx in the Uptake of Cell-Penetrating Peptides.
Attention for Chapter 6: Determining the Effects of Membrane-Interacting Peptides on Membrane Integrity.
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Chapter title
Determining the Effects of Membrane-Interacting Peptides on Membrane Integrity.
Chapter number 6
Book title
Cell-Penetrating Peptides
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2806-4_6
Pubmed ID
Book ISBNs
978-1-4939-2805-7, 978-1-4939-2806-4
Authors

Wimley, William C, William C. Wimley

Editors

Ülo Langel

Abstract

In the study of cell-penetrating and membrane-translocating peptides, a fundamental question occurs as to the contribution arising from fundamental peptide-membrane interactions, relative to the contribution arising from the biology and energy of the cell, mostly occurring in the form of endocytosis and subsequent events. A commonly used approach to begin addressing these mechanistic questions is to measure the degree to which peptides can interact with, and physically disrupt, the integrity of synthetic lipid bilayers. Here, we describe a set of experimental methods that can be used to measure the potency, kinetics, transience, and the effective size of peptide-induced membrane disruption.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 34%
Researcher 7 22%
Student > Doctoral Student 2 6%
Student > Postgraduate 2 6%
Professor 2 6%
Other 3 9%
Unknown 5 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 44%
Chemistry 6 19%
Agricultural and Biological Sciences 5 16%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Unknown 6 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 July 2015.
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#20,283,046
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Outputs from Methods in molecular biology
#9,911
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#295,832
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Outputs of similar age from Methods in molecular biology
#636
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