Chapter title |
Appendix A: Cryotech ® Vitrification Thawing
|
---|---|
Chapter number | 21 |
Book title |
Cryopreservation of Mammalian Gametes and Embryos
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6828-2_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6826-8, 978-1-4939-6828-2
|
Authors |
Goral Gandhi, Masashige Kuwayama, Sakina Kagalwala, Priyanka Pangerkar, Gandhi, Goral, Kuwayama, Masashige, Kagalwala, Sakina, Pangerkar, Priyanka |
Abstract |
In the last 15-20 years, many centers are using vitrification as a method of choice for cryopreservation of human oocytes and embryos. As vitrification technologies have improved their success profiles, new applications seem to have emerged, making IVF treatments more successful and more flexible.This appendix describes the Cryotech(®) method, which is the latest "minimal volume approach" method, suitable for cryopreservation of oocytes and embryos of any developmental stage, including blastocysts. Dr. Masashige Kuwayama, who has introduced major advances in oocyte and embryo cryopreservation, has developed this method. A detailed protocol has been described with finer tips for the accurate use of the method for perfect survival and safety. |
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