Cryopreservation of Mammalian Gametes and Embryos

James Graham, Josh Lim, Michael Tucker, Graham, James, Lim, Josh, Tucker, Michael

Improvements in oocyte cryopreservation has prompted wider acceptance of this technology leading to its use for several reasons. Notably, in addition to elective and medically driven reasons for oocyte cryostorage for fertility preservation, donor oocyte cryobanking is beginning to gain traction, potentially replacing fresh oocyte donation in assisted reproduction. Donor "egg banking," while not totally analogous to donor sperm banking, does provide strong benefits in terms of scheduling flexibility and improved clinical efficiencies, while providing a wider immediate inventory choice. The development of a successful cryobank "network" and subsequent growth into a full-access donor egg bank are only possible through adoption of a series of key steps involving establishment of a repeatable vitrification protocol with a strong clinical record, incorporation of a comprehensive database and quality management system, and strict control over the logistics of inventory and shipping and receiving, to establish a flawless chain from donor to recipient. Confidence will grow in this potentially difficult process over time.