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Cryopreservation of Mammalian Gametes and Embryos

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Cover of 'Cryopreservation of Mammalian Gametes and Embryos'

Table of Contents

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    Book Overview
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    Chapter 1 Chapter 1 Historical Background on Gamete and Embryo Cryopreservation
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    Chapter 2 Chapter 2 Utility of Animal Models for Human Ovarian Tissue Cryopreservation
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    Chapter 3 Chapter 3 Current Challenges in Immature Oocyte Cryopreservation
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    Chapter 4 Chapter 4 Role of Antioxidants and Antifreeze Proteins in Cryopreservation/Vitrification
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    Chapter 5 Chapter 5 Slow Freezing of Human Sperm
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    Chapter 6 Chapter 6 Technology of Aseptic Cryoprotectant-Free Vitrification of Human ICSI Spermatozoa
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    Chapter 7 Chapter 7 Human Epididymal and Testicular Sperm Cryopreservation
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    Chapter 8 Chapter 8 Human Oocytes Slow-Rate Freezing: Methodology
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    Chapter 9 Chapter 9 Slow Freezing and Thawing of Human Cleavage Stage Embryos
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    Chapter 10 Chapter 10 Human Oocyte Vitrification
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    Chapter 11 Chapter 11 Human Embryo Vitrification
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    Chapter 12 Chapter 12 Human Ovarian Tissue Slow Freezing
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    Chapter 13 Chapter 13 Human Ovarian Tissue Vitrification
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    Chapter 14 Chapter 14 Establishing an Oocyte Cryobank Network
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    Chapter 15 Chapter 15 Development of a Nationwide Network for Ovarian Tissue Cryopreservation
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    Chapter 16 Chapter 16 Directional Freezing of Ovarian Tissue and Freeze-Drying of Stem Cells for Fertility Preservation
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    Chapter 17 Chapter 17 Sterile Plate-Based Vitrification of Adherent Human Pluripotent Stem Cells and Their Derivatives Using the TWIST Method
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    Chapter 18 Chapter 18 Vitrification: A Reliable Method for Cryopreservation of Animal Embryos
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    Chapter 19 Chapter 19 Cryopreservation Effect on Genetic Function: Neonatal Outcomes
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    Chapter 20 Chapter 20 Gavi-Automated Vitrification Instrument
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    Chapter 21 Appendix A: Cryotech ® Vitrification Thawing
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    Chapter 22 Appendix B: Solid Surface Vitrification
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    Chapter 23 Appendix C: Automated Vitrification of Mammalian Embryos on a Digital Microfluidic Device
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    Chapter 24 Appendix D: Irvine Scientific ® Vitrification System
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    Chapter 25 Appendix E: Rapid-i TM : Closed Vitrification Device by Vitrolife
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    Chapter 26 Appendix F: Quinn’s Advantage Embryo Freeze Kit
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    Chapter 27 Appendix G: Vitrification of Blastocysts Using VitriBlast TM and ThermoBlast TM : Nidacon
Attention for Chapter 6: Chapter 6 Technology of Aseptic Cryoprotectant-Free Vitrification of Human ICSI Spermatozoa
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Chapter title
Chapter 6 Technology of Aseptic Cryoprotectant-Free Vitrification of Human ICSI Spermatozoa
Chapter number 6
Book title
Cryopreservation of Mammalian Gametes and Embryos
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6828-2_6
Pubmed ID
Book ISBNs
978-1-4939-6826-8, 978-1-4939-6828-2
Authors

Vladimir Isachenko, Raul Sanchez, Peter Mallmann, Gohar Rahimi, Evgenia Isachenko, Isachenko, Vladimir, Sanchez, Raul, Mallmann, Peter, Rahimi, Gohar, Isachenko, Evgenia

Abstract

The aim of this chapter was to describe the standardized aseptic technology of permeable cryoprotectant-free vitrification of human spermatozoa in capillaries (for ICSI or IVF in microvolume). Spermatozoa, vitrified by this technology, are free from seminal plasma owing to swim-up procedure preceding vitrification and are free from permeable cryoprotectants. They are ready for further use immediately after warming without any additional treatment.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 60%
Student > Bachelor 1 20%
Unknown 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 40%
Medicine and Dentistry 2 40%
Computer Science 1 20%