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Chapter title |
Chapter 6 Technology of Aseptic Cryoprotectant-Free Vitrification of Human ICSI Spermatozoa
|
---|---|
Chapter number | 6 |
Book title |
Cryopreservation of Mammalian Gametes and Embryos
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6828-2_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6826-8, 978-1-4939-6828-2
|
Authors |
Vladimir Isachenko, Raul Sanchez, Peter Mallmann, Gohar Rahimi, Evgenia Isachenko, Isachenko, Vladimir, Sanchez, Raul, Mallmann, Peter, Rahimi, Gohar, Isachenko, Evgenia |
Abstract |
The aim of this chapter was to describe the standardized aseptic technology of permeable cryoprotectant-free vitrification of human spermatozoa in capillaries (for ICSI or IVF in microvolume). Spermatozoa, vitrified by this technology, are free from seminal plasma owing to swim-up procedure preceding vitrification and are free from permeable cryoprotectants. They are ready for further use immediately after warming without any additional treatment. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 60% |
Student > Bachelor | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 40% |
Medicine and Dentistry | 2 | 40% |
Computer Science | 1 | 20% |