Chapter title |
Chapter 8 Human Oocytes Slow-Rate Freezing: Methodology
|
---|---|
Chapter number | 8 |
Book title |
Cryopreservation of Mammalian Gametes and Embryos
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6828-2_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6826-8, 978-1-4939-6828-2
|
Authors |
C. Zacà, A. Borini, Zacà, C., Borini, A. |
Abstract |
Cryopreservation of human oocytes is an important technique for the treatment of human infertility, as it deals successfully with legal, ethical, and moral issues related to embryo cryopreservation (Coticchio et al., Human Fertil (Camb) 4:152-157, 2001; Tucker et al., Eur J Obstet Gynecol Reprod Biol 113: 524-527, 2004) and maintains reproductive potential in women with diseases or conditions that may compromise reproductive capacity. Here, we describe an oocyte cryopreservation technique involving slow freezing-rapid thawing with differential sucrose concentration (0.2 M in the freezing stage-0.3 M in thawing stage) (Bianchi et al., Reprod Biomed Online. 14:64-71, 2007), describing the technique in detail, from the preparation of the solutions through to the performance of the technique and, finally, to a number of helpful hints for optimum results. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 29% |
Student > Bachelor | 1 | 14% |
Other | 1 | 14% |
Student > Master | 1 | 14% |
Student > Ph. D. Student | 1 | 14% |
Other | 0 | 0% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 4 | 57% |
Arts and Humanities | 1 | 14% |
Agricultural and Biological Sciences | 1 | 14% |
Biochemistry, Genetics and Molecular Biology | 1 | 14% |