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Mendeley readers
| Chapter title |
Screening of Kinase Substrates Using Kinase Knockout Mutants
|
|---|---|
| Chapter number | 4 |
| Book title |
Plant Phosphoproteomics
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2648-0_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2647-3, 978-1-4939-2648-0
|
| Authors |
Taishi Umezawa, Umezawa, Taishi |
| Abstract |
Protein kinases are widely known to be major regulators of various signaling processes, particularly in eukaryotes, including plants. To understand their role in signal transduction pathways, it is necessary to determine which proteins are phosphorylated by these enzymes. Recent studies have applied a comparative phosphoproteomic approach to identify protein kinase substrates in plants. The results demonstrated that kinase knockout mutants are useful for screening protein kinase substrates via such a comparative analysis. Here some technical points are described for the experimental design and comparative analysis using kinase knockout mutants. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 33% |
| Unknown | 2 | 67% |
| Readers by discipline | Count | As % |
|---|---|---|
| Computer Science | 1 | 33% |
| Unknown | 2 | 67% |