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Plant Phosphoproteomics

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Cover of 'Plant Phosphoproteomics'

Table of Contents

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    Book Overview
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    Chapter 1 The Plant Kinome
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    Chapter 2 Phosphatases in plants.
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    Chapter 3 Phosphoproteomics in cereals.
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    Chapter 4 Screening of Kinase Substrates Using Kinase Knockout Mutants
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    Chapter 5 Phosphopeptide Profiling of Receptor Kinase Mutants
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    Chapter 6 Combining Metabolic (15)N Labeling with Improved Tandem MOAC for Enhanced Probing of the Phosphoproteome.
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    Chapter 7 Kinase activity and specificity assay using synthetic peptides.
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    Chapter 8 Absolute quantitation of protein posttranslational modification isoform.
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    Chapter 9 Phosphorylation Stoichiometry Determination in Plant Photosynthetic Membranes
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    Chapter 10 Phosphopeptide immuno-affinity enrichment to enhance detection of tyrosine phosphorylation in plants.
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    Chapter 11 The Peptide Microarray ChloroPhos1.0: A Screening Tool for the Identification of Arabidopsis thaliana Chloroplast Protein Kinase Substrates
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    Chapter 12 Plant Protein Kinase Substrates Identification Using Protein Microarrays
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    Chapter 13 Targeted Analysis of Protein Phosphorylation by 2D Electrophoresis.
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    Chapter 14 Computational phosphorylation network reconstruction: methods and resources.
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    Chapter 15 Computational Identification of Protein Kinases and Kinase-Specific Substrates in Plants
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    Chapter 16 Databases for plant phosphoproteomics.
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    Chapter 17 Phosphorylation Site Prediction in Plants
Attention for Chapter 12: Plant Protein Kinase Substrates Identification Using Protein Microarrays
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Chapter title
Plant Protein Kinase Substrates Identification Using Protein Microarrays
Chapter number 12
Book title
Plant Phosphoproteomics
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2648-0_12
Pubmed ID
Book ISBNs
978-1-4939-2647-3, 978-1-4939-2648-0
Authors

Shisong Ma, Savithramma P. Dinesh-Kumar, Ma, Shisong, Dinesh-Kumar, Savithramma P.

Abstract

Protein kinases regulate signaling pathways by phosphorylating their targets. They play critical roles in plant signaling networks. Although many important protein kinases have been identified in plants, their substrates are largely unknown. We have developed and produced plant protein microarrays with more than 15,000 purified plant proteins. Here, we describe a detailed protocol to use these microarrays to identify plant protein kinase substrates via in vitro phosphorylation assays on these arrays.

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The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 50%
Student > Ph. D. Student 1 25%
Student > Master 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 75%
Computer Science 1 25%