Chapter title |
Studying the Functions of TGF-β Signaling in the Ovary.
|
---|---|
Chapter number | 19 |
Book title |
TGF-β Signaling
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2966-5_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2965-8, 978-1-4939-2966-5
|
Authors |
Yu, Chao, Zhou, Jian-Jie, Fan, Heng-Yu, Chao Yu, Jian-Jie Zhou, Heng-Yu Fan |
Abstract |
In mammals, ovulation is a multistep physiological process that includes preovulatory follicle growth, oocyte meiotic maturation, cumulus-oocyte complex (COC) expansion, follicle rupture, and luteinization. TGF-β signaling pathway has multiple functions in mammalian ovary, as its complexity in ovarian function has been demonstrated by mouse models with knockouts of TGF-β receptors and SMADs. We describe the protocol that we use to study functions of TGF-β signaling pathway in follicle development and ovulation. Because total knockout of TGF-β pathway components often causes embryonic lethality, which prevents further investigation of these genes in ovarian functions, people have generated ovarian cell type-specific knockout mouse strains for TGF-β signaling pathway genes. These mouse models are also described. |
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