Chapter title |
Electrotonic Coupled Metabolic Purification of Chick Cardiomyocytes
|
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Chapter number | 14 |
Book title |
Cardiomyocytes
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2572-8_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2571-1, 978-1-4939-2572-8
|
Authors |
Winston Shim, Haiyang Yu, K. P. Myu Mai Ja, Muhammad Parasuram, Kee Pah Lim, Philip Wong, Shim, Winston, Yu, Haiyang, Ja, K. P. Myu Mai, Parasuram, Muhammad, Lim, Kee Pah, Wong, Philip |
Abstract |
Cardiomyocytes isolated from chick and rodent are widely used in studying cardiac physiology. However, contaminating non-cardiomyocytes are an inherent problem that hinders downstream analysis. Here, we report a novel electrical stimulation coupled with metabolic selection method using cytosine arabinoside (AraC) to efficiently eliminate contaminating cells in isolating chick embryonic cardiomyocytes. Compared with conventional methods of pre-plating or AraC alone, electrical stimulation coupled with AraC increased the percentage purity of cardiomyocytes by 2-6-fold with added effect of improved contractile function and maturation. This simple method could be useful in isolating and maintaining purified cardiomyocytes for long-term studies of cardiac physiology. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 100% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 50% |
Unknown | 1 | 50% |