Chapter title |
Enrichment of Cardiomyocytes in Primary Cultures of Murine Neonatal Hearts
|
---|---|
Chapter number | 2 |
Book title |
Cardiomyocytes
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2572-8_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2571-1, 978-1-4939-2572-8
|
Authors |
Sreejit Parameswaran, Rajalakshmi Santhakumar, Prasanna Vidyasekar, Rama S. Verma, Parameswaran, Sreejit, Santhakumar, Rajalakshmi, Vidyasekar, Prasanna, Verma, Rama S. |
Abstract |
In vitro culture of neonatal murine cardiomyocytes is vital for understanding the functions of the heart. Cardiomyocyte cultures are difficult to maintain because they do not proliferate after birth. The maintenance of primary cultures of viable and functional cardiomyocytes is considerably affected by the yield from initial steps of isolation procedures. This protocol describes an efficient and rapid method for isolation and maintenance of long-term cultures of neonatal murine cardiomyocytes by effectively shortening the trypsin enzyme digestion period and the cardiomyocyte enrichment step. |
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Geographical breakdown
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Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 20% |
Student > Ph. D. Student | 2 | 20% |
Other | 1 | 10% |
Professor | 1 | 10% |
Other | 0 | 0% |
Unknown | 1 | 10% |
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Earth and Planetary Sciences | 1 | 10% |
Engineering | 1 | 10% |
Other | 0 | 0% |
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