| Chapter title |
A Homokaryon Assay for Nucleocytoplasmic Shuttling Activity of HBV Core Protein
|
|---|---|
| Chapter number | 5 |
| Book title |
Hepatitis B Virus
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6700-1_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6698-1, 978-1-4939-6700-1
|
| Authors |
Ching-Chun Yang, Hung-Cheng Li, Chiaho Shih |
| Abstract |
Hepatitis B virus (HBV) core protein (HBc) can be present in both nucleus and cytoplasm. The arginine-rich domain (ARD) at the cytoplasmic tail of HBc contains both a nuclear localization signal (NLS) and nuclear export signal (NES). We established a homokaryon assay to detect the dynamic trafficking of HBc between nucleus and cytoplasm in hepatocytes. Using immunofluorescence assay (IFA) and PEG-induced cell-cell fusion, we demonstrated that a chimeric reporter protein of SV40 large T antigen, when fused in-frame with HBc ARD, can shuttle from a donor nuclei (green) to the recipient nuclei (red) in the context of binucleated or polynucleated hybrid cells. The shuttling activity driven by HBc ARD can be measured quantitatively by this IFA method. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 1 | 25% |
| Unknown | 3 | 75% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemical Engineering | 1 | 25% |
| Immunology and Microbiology | 1 | 25% |
| Unknown | 2 | 50% |