Chapter title |
Measuring Changes in Cytosolic Calcium Levels in HBV- and HBx-Expressing Cultured Primary Hepatocytes
|
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Chapter number | 12 |
Book title |
Hepatitis B Virus
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6700-1_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6698-1, 978-1-4939-6700-1
|
Authors |
Jessica C. Casciano, Michael J. Bouchard |
Abstract |
Chronic infection with hepatitis B virus (HBV) remains a major worldwide health concern and is the leading cause of hepatocellular carcinoma (HCC). The HBV X protein (HBx) is the only regulatory protein encoded in the HBV genome; HBx stimulates HBV replication in vivo and in vitro. HBx also regulates cytosolic Ca(2+) signaling, and altered Ca(2+) signaling is associated with the development of many diseases, including HCC. Importantly, many HBx functions, including HBx modulation of cell proliferation, apoptosis, and transcription pathways, have been linked to changes in cytosolic Ca(2+) signaling. Additionally, several stages of HBV replication, including capsid formation and activation of the HBV polymerase, are dependent on intracellular Ca(2+). Consequently, defining the molecular mechanism that underlies HBV and HBx modulation of cytosolic Ca(2+) levels is important for understanding HBV pathogenesis and the role of HBx in HBV replication. Here, we describe a single-cell Ca(2+)-imaging protocol that we use to investigate HBV and HBx effects on the level of cytosolic Ca(2+). We specifically outline two methods that we use to evaluate HBV and HBx regulation of cytosolic Ca(2+) levels in cultured primary hepatocytes. This protocol can also be adapted for use in liver cell lines. |
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