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Hepatitis B Virus

Overview of attention for book
Cover of 'Hepatitis B Virus'

Table of Contents

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    Book Overview
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    Chapter 1 NTCP-Reconstituted In Vitro HBV Infection System
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    Chapter 2 Hepatitis B Virus Infection of HepaRG Cells, HepaRG-hNTCP Cells, and Primary Human Hepatocytes
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    Chapter 3 Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells
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    Chapter 4 Intracytoplasmic Transport of Hepatitis B Virus Capsids
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    Chapter 5 A Homokaryon Assay for Nucleocytoplasmic Shuttling Activity of HBV Core Protein
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    Chapter 6 Analyses of HBV cccDNA Quantification and Modification
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    Chapter 7 Detection of HBV cccDNA Methylation from Clinical Samples by Bisulfite Sequencing and Methylation-Specific PCR
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    Chapter 8 A T7 Endonuclease I Assay to Detect Talen-Mediated Targeted Mutation of HBV cccDNA
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    Chapter 9 Detection of Hepatocyte Clones Containing Integrated Hepatitis B Virus DNA Using Inverse Nested PCR
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    Chapter 10 Highly Sensitive Detection of HBV RNA in Liver Tissue by In Situ Hybridization
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    Chapter 11 Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice
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    Chapter 12 Measuring Changes in Cytosolic Calcium Levels in HBV- and HBx-Expressing Cultured Primary Hepatocytes
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    Chapter 13 In Vitro Assays for RNA Binding and Protein Priming of Hepatitis B Virus Polymerase
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    Chapter 14 In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV RNaseH Inhibitors
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    Chapter 15 Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay
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    Chapter 16 Microtiter-Format Assays for HBV Antigen Quantitation in Nonclinical Applications
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    Chapter 17 Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform
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    Chapter 18 Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
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    Chapter 19 Hydrodynamic HBV Transfection Mouse Model
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    Chapter 20 An ELISPOT-Based Assay to Measure HBV-Specific CD8 + T Cell Responses in Immunocompetent Mice
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    Chapter 21 Advanced Method for Isolation of Mouse Hepatocytes, Liver Sinusoidal Endothelial Cells, and Kupffer Cells
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    Chapter 22 Partial Hepatectomy and Castration of HBV Transgenic Mice
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    Chapter 23 Studying HBV Infection and Therapy in Immune-Deficient NOD-Rag1−/−IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (Fah) Knockout Mice Transplanted with Human Hepatocytes
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    Chapter 24 Measurement of Antiviral Effect and Innate Immune Response During Treatment of Primary Woodchuck Hepatocytes
Attention for Chapter 1: NTCP-Reconstituted In Vitro HBV Infection System
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Chapter title
NTCP-Reconstituted In Vitro HBV Infection System
Chapter number 1
Book title
Hepatitis B Virus
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6700-1_1
Pubmed ID
27975303
Book ISBNs
978-1-4939-6698-1, 978-1-4939-6700-1
Authors

Yinyan Sun, Yonghe Qi, Bo Peng, Wenhui Li

Abstract

Sodium taurocholate cotransporting polypeptide (NTCP) has been identified as a functional receptor for hepatitis B virus (HBV). Expressing human NTCP in human hepatoma HepG2 cells (HepG2-NTCP) renders these cells susceptible for HBV infection. The HepG2-NTCP stably transfected cell line provides a much-needed and easily accessible platform for studying the virus. HepG2-NTCP cells could also be used to identify chemicals targeting key steps of the virus life cycle including HBV covalent closed circular (ccc) DNA, and enable the development of novel antivirals against the infection.Many factors may contribute to the efficiency of HBV infection on HepG2-NTCP cells, with clonal differences among cell line isolates, the source of viral inoculum, and infection medium among the most critical ones. Here, we provide detailed protocols for efficient HBV infection of HepG2-NTCP cells in culture; generation and selection of single cell clones of HepG2-NTCP; production of infectious HBV virion stock through DNA transfection of recombinant plasmid that enables studying primary clinical HBV isolates; and assessing the infection with immunostaining of HBV antigens and Southern blot analysis of HBV cccDNA.

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Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 18%
Student > Ph. D. Student 3 14%
Student > Postgraduate 2 9%
Researcher 2 9%
Other 1 5%
Other 2 9%
Unknown 8 36%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 18%
Pharmacology, Toxicology and Pharmaceutical Science 2 9%
Immunology and Microbiology 2 9%
Agricultural and Biological Sciences 2 9%
Medicine and Dentistry 2 9%
Other 2 9%
Unknown 8 36%

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