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Polyadenylation in Plants

Overview of attention for book
Cover of 'Polyadenylation in Plants'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Computational Analysis of Plant Polyadenylation Signals
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    Chapter 2 Prediction of Plant mRNA Polyadenylation Sites
  4. Altmetric Badge
    Chapter 3 Extraction of Poly(A) Sites from Large-Scale RNA-seq Data.
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    Chapter 4 Poly(A)-Tag Deep Sequencing Data Processing to Extract Poly(A) Sites
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    Chapter 5 Analysis of Poly(A) Site Choice Using a Java-Based Clustering Algorithm
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    Chapter 6 RADPRE: A Computational Program for Identification of Differential mRNA Processing Including Alternative Polyadenylation
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    Chapter 7 Characterization of plant polyadenylation complexes by using tandem affinity purification.
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    Chapter 8 In vitro analysis of cleavage and polyadenylation in Arabidopsis.
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    Chapter 9 Production, Purification, and Assay of Recombinant Proteins for In Vitro Biochemical Analyses of the Plant Polyadenylation Complex
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    Chapter 10 Detection of Disulfide Linkage by Chemical Derivatization and Mass Spectrometry
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    Chapter 11 Transient Expression Using Agroinfiltration to Study Polyadenylation in Plants
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    Chapter 12 A 3′ RACE Protocol to Confirm Polyadenylation Sites
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    Chapter 13 Phage Display Library Screening for Identification of Interacting Protein Partners
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    Chapter 14 Genome-Wide Determination of Poly(A) Site Choice in Plants
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    Chapter 15 DNA/RNA Hybrid Primer Mediated Poly(A) Tag Library Construction for Illumina Sequencing
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    Chapter 16 Poly(A) Tag Library Construction from 10 ng Total RNA.
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    Chapter 17 A Rapid, Simple, and Inexpensive Method for the Preparation of Strand-Specific RNA-Seq Libraries
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    Chapter 18 Genome-Wide Analysis of Distribution of RNA Polymerase II Isoforms Using ChIP-Seq.
Attention for Chapter 16: Poly(A) Tag Library Construction from 10 ng Total RNA.
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Chapter title
Poly(A) Tag Library Construction from 10 ng Total RNA.
Chapter number 16
Book title
Polyadenylation in Plants
Published in
Methods in molecular biology, December 2014
DOI 10.1007/978-1-4939-2175-1_16
Pubmed ID
Book ISBNs
978-1-4939-2174-4, 978-1-4939-2175-1

Cao J, Li QQ, Jingyi Cao, Qingshun Quinn Li, Cao, Jingyi, Li, Qingshun Quinn


Alternative polyadenylation has been demonstrated as a tier of gene expression regulation in eukaryotes. However, its role has not been elucidated at the cellular level. Equipped with techniques to isolate single cells by fluorescence-activated cell sorting (FACS) and laser captured micro-dissection, analysis of alternative polyadenylation in specific cell types becomes possible. We present a method to generate poly(A) tags for high-throughput sequencing (PAT-seq) libraries from very low amount of total RNA. This protocol targets the junction of the 3'-UTR and poly(A) tail of transcripts. Ten nanograms of total RNA isolated from the FACS-sorted cells was reverse-transcribed to double stranded cDNA with a anchored oligo dT(18) primer containing maximal T7 promoter sequence. Then, an RNA amplification step using in vitro transcription of T7 RNA polymerase was carried out. Achieved cRNA was fragmented by partial digestion. First strand synthesis was carried out by using a partial adaptor sequence with random 9-nt primer to introduce the adaptor at the 5' end. An anchored oligo dT primer containing adaptor sequence on 3' end was introduced through second strand cDNA synthesis. This new method has been applied to investigate polyadenylation using nanogram amount of total RNA from Arabidopsis cells.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
South Africa 1 17%
Unknown 5 83%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 33%
Other 1 17%
Librarian 1 17%
Student > Master 1 17%
Student > Ph. D. Student 1 17%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 50%
Arts and Humanities 1 17%
Biochemistry, Genetics and Molecular Biology 1 17%
Medicine and Dentistry 1 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2014.
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Outputs of similar age from Methods in molecular biology
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Altmetric has tracked 22,774,233 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,091 research outputs from this source. They receive a mean Attention Score of 3.3. This one has gotten more attention than average, scoring higher than 64% of its peers.
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We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.