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Polyadenylation in Plants

Overview of attention for book
Cover of 'Polyadenylation in Plants'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Computational Analysis of Plant Polyadenylation Signals
  3. Altmetric Badge
    Chapter 2 Prediction of Plant mRNA Polyadenylation Sites
  4. Altmetric Badge
    Chapter 3 Extraction of Poly(A) Sites from Large-Scale RNA-seq Data.
  5. Altmetric Badge
    Chapter 4 Poly(A)-Tag Deep Sequencing Data Processing to Extract Poly(A) Sites
  6. Altmetric Badge
    Chapter 5 Analysis of Poly(A) Site Choice Using a Java-Based Clustering Algorithm
  7. Altmetric Badge
    Chapter 6 RADPRE: A Computational Program for Identification of Differential mRNA Processing Including Alternative Polyadenylation
  8. Altmetric Badge
    Chapter 7 Characterization of plant polyadenylation complexes by using tandem affinity purification.
  9. Altmetric Badge
    Chapter 8 In vitro analysis of cleavage and polyadenylation in Arabidopsis.
  10. Altmetric Badge
    Chapter 9 Production, Purification, and Assay of Recombinant Proteins for In Vitro Biochemical Analyses of the Plant Polyadenylation Complex
  11. Altmetric Badge
    Chapter 10 Detection of Disulfide Linkage by Chemical Derivatization and Mass Spectrometry
  12. Altmetric Badge
    Chapter 11 Transient Expression Using Agroinfiltration to Study Polyadenylation in Plants
  13. Altmetric Badge
    Chapter 12 A 3′ RACE Protocol to Confirm Polyadenylation Sites
  14. Altmetric Badge
    Chapter 13 Phage Display Library Screening for Identification of Interacting Protein Partners
  15. Altmetric Badge
    Chapter 14 Genome-Wide Determination of Poly(A) Site Choice in Plants
  16. Altmetric Badge
    Chapter 15 DNA/RNA Hybrid Primer Mediated Poly(A) Tag Library Construction for Illumina Sequencing
  17. Altmetric Badge
    Chapter 16 Poly(A) Tag Library Construction from 10 ng Total RNA.
  18. Altmetric Badge
    Chapter 17 A Rapid, Simple, and Inexpensive Method for the Preparation of Strand-Specific RNA-Seq Libraries
  19. Altmetric Badge
    Chapter 18 Genome-Wide Analysis of Distribution of RNA Polymerase II Isoforms Using ChIP-Seq.
Attention for Chapter 17: A Rapid, Simple, and Inexpensive Method for the Preparation of Strand-Specific RNA-Seq Libraries
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)
  • High Attention Score compared to outputs of the same age and source (95th percentile)

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1 blog
twitter
1 tweeter
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1 Google+ user

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15 Mendeley
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Chapter title
A Rapid, Simple, and Inexpensive Method for the Preparation of Strand-Specific RNA-Seq Libraries
Chapter number 17
Book title
Polyadenylation in Plants
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2175-1_17
Pubmed ID
Book ISBNs
978-1-4939-2174-4, 978-1-4939-2175-1
Authors

Arthur G Hunt, Arthur G. Hunt, Hunt, Arthur G.

Abstract

High-throughput sequencing of short cDNA tags, or RNA-Seq, has become a staple of genome-wide gene expression studies in plants. RNA-Seq libraries necessarily contain tags that correspond to the mRNA-poly(A) junction, or polyadenylation site, and thus may be mined for data that can help study alternative polyadenylation. This report presents a simple, rapid, and inexpensive method for preparing strand-specific RNA-Seq libraries from varying quantities of total RNA.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 7%
Denmark 1 7%
Unknown 13 87%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 53%
Researcher 2 13%
Librarian 1 7%
Student > Bachelor 1 7%
Lecturer 1 7%
Other 2 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 67%
Biochemistry, Genetics and Molecular Biology 3 20%
Arts and Humanities 1 7%
Mathematics 1 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2014.
All research outputs
#2,549,397
of 16,638,522 outputs
Outputs from Methods in molecular biology
#545
of 9,651 outputs
Outputs of similar age
#46,137
of 310,857 outputs
Outputs of similar age from Methods in molecular biology
#36
of 883 outputs
Altmetric has tracked 16,638,522 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,651 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 310,857 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 883 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.