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Polyadenylation in Plants

Overview of attention for book
Cover of 'Polyadenylation in Plants'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Computational Analysis of Plant Polyadenylation Signals
  3. Altmetric Badge
    Chapter 2 Prediction of Plant mRNA Polyadenylation Sites
  4. Altmetric Badge
    Chapter 3 Extraction of Poly(A) Sites from Large-Scale RNA-seq Data.
  5. Altmetric Badge
    Chapter 4 Poly(A)-Tag Deep Sequencing Data Processing to Extract Poly(A) Sites
  6. Altmetric Badge
    Chapter 5 Analysis of Poly(A) Site Choice Using a Java-Based Clustering Algorithm
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    Chapter 6 RADPRE: A Computational Program for Identification of Differential mRNA Processing Including Alternative Polyadenylation
  8. Altmetric Badge
    Chapter 7 Characterization of plant polyadenylation complexes by using tandem affinity purification.
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    Chapter 8 In vitro analysis of cleavage and polyadenylation in Arabidopsis.
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    Chapter 9 Production, Purification, and Assay of Recombinant Proteins for In Vitro Biochemical Analyses of the Plant Polyadenylation Complex
  11. Altmetric Badge
    Chapter 10 Detection of Disulfide Linkage by Chemical Derivatization and Mass Spectrometry
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    Chapter 11 Transient Expression Using Agroinfiltration to Study Polyadenylation in Plants
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    Chapter 12 A 3′ RACE Protocol to Confirm Polyadenylation Sites
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    Chapter 13 Phage Display Library Screening for Identification of Interacting Protein Partners
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    Chapter 14 Genome-Wide Determination of Poly(A) Site Choice in Plants
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    Chapter 15 DNA/RNA Hybrid Primer Mediated Poly(A) Tag Library Construction for Illumina Sequencing
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    Chapter 16 Poly(A) Tag Library Construction from 10 ng Total RNA.
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    Chapter 17 A Rapid, Simple, and Inexpensive Method for the Preparation of Strand-Specific RNA-Seq Libraries
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    Chapter 18 Genome-Wide Analysis of Distribution of RNA Polymerase II Isoforms Using ChIP-Seq.
Attention for Chapter 8: In vitro analysis of cleavage and polyadenylation in Arabidopsis.
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Chapter title
In vitro analysis of cleavage and polyadenylation in Arabidopsis.
Chapter number 8
Book title
Polyadenylation in Plants
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2175-1_8
Pubmed ID
25487206
Book ISBNs
978-1-4939-2174-4, 978-1-4939-2175-1
Authors

Hongwei Zhao, Qingshun Quinn Li, Zhao, Hongwei, Li, Qingshun Quinn

Abstract

In eukaryotes, pre-messenger RNA (pre-mRNA) cleavage and polyadenylation is one of the necessary processing steps that produce a mature and functional mRNA. Regulation on pre-mRNA cleavage and polyadenylation affects other processes such as mRNA translocation, stability, and translation. The process of pre-mRNA cleavage and polyadenylation, and its relationship with RNA splicing and translation, have been extensively studied due to its importance in vivo. A successful in vitro system has provided enormous amount of information to the study of cleavage and polyadenylation in the mammalian and yeast systems. Here, we describe an in vitro pre-mRNA cleavage system that faithfully cleaves pre-mRNA substrate using Arabidopsis cell/tissue cultures.

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Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 50%
Researcher 1 50%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 December 2014.
All research outputs
#20,246,428
of 22,774,233 outputs
Outputs from Methods in molecular biology
#9,866
of 13,091 outputs
Outputs of similar age
#295,638
of 352,917 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
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