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Mechanisms of Lymphocyte Activation and Immune Regulation VI

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Cover of 'Mechanisms of Lymphocyte Activation and Immune Regulation VI'

Table of Contents

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    Book Overview
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    Chapter 1 Apoptosis/Programmed Cell Death
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    Chapter 2 Apoptosis and Its Regulation
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    Chapter 3 Mechanisms for Recognition and Phagocytosis of Apoptotic Lymphocytes by Macrophages
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    Chapter 4 Cytotoxic Lymphocyte Killing Enters the Ice Age
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    Chapter 5 Cross-talk between ceramide and PKC activity in the control of apoptosis in WEHI-231.
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    Chapter 6 Cell Cycle Control of T Cell Apoptosis Induced by Activation Through the T Cell Antigen Receptor
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    Chapter 7 Role of antibody signaling in inducing tumor dormancy.
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    Chapter 8 Regulation of lymphoid apoptosis by Bcl-2 and Bcl-XL.
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    Chapter 9 The Epstein-Barr virus gene BHRF1, a homologue of the cellular oncogene Bcl-2, inhibits apoptosis induced by gamma radiation and chemotherapeutic drugs.
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    Chapter 10 Structure—Function Analysis of Bcl-2 Family Proteins
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    Chapter 11 Role of Ice-Proteases in Apoptosis
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    Chapter 12 Fas-mediated apoptosis.
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    Chapter 13 Fas Splicing Variants and their Effect on Apoptosis
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    Chapter 14 The Role of FasL and TNF in the Homeostatic Regulation of Immune Responses
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    Chapter 15 Signals for survival and apoptosis in normal and neoplastic B lymphocytes.
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    Chapter 16 Regulation of B Cell Apoptosis
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    Chapter 17 Apoptotic Cell Death in the Chicken Bursa of Fabricius
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    Chapter 18 Generation and Regulation of B Cell Autoreactivity Arising in the Periphery
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    Chapter 19 Inducible Resistance to Fas-Mediated Apoptosis in Primary B Lymphocytes
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    Chapter 20 The thymus and T cell death.
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    Chapter 21 Genetic Regulation of Apoptosis in the Mouse Thymus
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    Chapter 22 Regulation of T Cell Activation by CD28 and CTLA4
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    Chapter 23 Granzyme B-Induced Apoptosis
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    Chapter 24 Mature T Lymphocyte Apoptosis in the Healthy and Diseased Immune System
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    Chapter 25 Autoimmunity Due to Defective NUR77, Fas, and TNF-RI Apoptosis
Attention for Chapter 5: Cross-talk between ceramide and PKC activity in the control of apoptosis in WEHI-231.
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Chapter title
Cross-talk between ceramide and PKC activity in the control of apoptosis in WEHI-231.
Chapter number 5
Book title
Mechanisms of Lymphocyte Activation and Immune Regulation VI
Published in
Advances in experimental medicine and biology, January 1996
DOI 10.1007/978-1-4899-0274-0_5
Pubmed ID
Book ISBNs
978-1-4899-0276-4, 978-1-4899-0274-0

Chmura, S J, Nodzenski, E, Crane, M A, Virudachalam, S, Hallahan, D E, Weichselbaum, R R, Quintans, J, Chmura, Steven J., Nodzenski, Edwardine, Crane, Mary A., Virudachalam, Subbulakshmi, Hallahan, Dennis E., Weichselbaum, Ralph R., Quintans, Jose


WEHI-231, a murine B-cell lymphoma, readily undergoes programmed cell death following surface immunoglobulin (Ig) cross-linking [1]. Ceramide has been shown to induce apoptosis in WEHI-231 following its exposure to anti-lg antibodies, dexamethasone, and irradiation [2]. Recently, Haimovitz-Friedman et al. have demonstrated in endothelial cells that PMA not only prevented ceramide mediated apoptosis, but inhibited the generation of ceramide following irradiation [3]. In this paper we use highly specific PKC inhibitors to explore the connection between PKC activity, ceramide signaling and apoptosis. Both chelerythrine chloride and calphostin C triggered rapid apoptosis in WEHI-231 and acted in synergy with exogenous ceramide to induce apoptosis. Detailed studies of chelerythrine's mechanism of action revealed that 30 minutes following addition of 10 microM chelerythrine, sphingomyelin and phosphatidylcholine (PC) mass decreased confirming our previous findings of neutral, but not acidic, sphingomyelinase activation following treatment with PKC inhibitors [4]. The novel observation that inhibition of PKC isoforms present in WEHI-231 leads to a rapid rise in cellular ceramide as a results of sphingomyelin hydrolysis further suggests an antagonistic relationship between PKC activity and ceramide in the signaling events preceding apoptosis.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 25%
Unknown 3 75%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 2 50%
Professor 1 25%
Student > Ph. D. Student 1 25%
Researcher 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 50%
Medicine and Dentistry 2 50%
Immunology and Microbiology 1 25%