Chapter title |
Structure, Mechanism, and Mutation of Bacterial Luciferase
|
---|---|
Chapter number | 281 |
Book title |
Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3
|
Published in |
Advances in biochemical engineering biotechnology, December 2014
|
DOI | 10.1007/10_2014_281 |
Pubmed ID | |
Book ISBNs |
978-3-31-927405-8, 978-3-31-927407-2
|
Authors |
Ruchanok Tinikul, Pimchai Chaiyen, Tinikul, Ruchanok, Chaiyen, Pimchai |
Abstract |
: Bacterial luciferase is a flavin-dependent monooxygenase found in bioluminescent bacteria. The enzyme catalyzes a light-emitting reaction by using reduced flavin, long chain aldehyde, and oxygen as substrates and yields oxidized flavin, carboxylic acid, and water as products with concomitant emission of blue-green light around 485-490 nm. The enzyme is a heterodimer consisting of two homologous subunits, designated as the α- and β-subunits. The reactive reaction center is located in the α-subunit, whereas the β-subunit is required for maintaining the active conformation of the α-subunit. The enzyme reaction occurs through the generation of a reactive C4a-oxygenflavin adduct, presumably C4a-peroxyflavin, before the light-emitting species is generated from the decomposition of an adduct between the C4a-peroxyflavin and the aldehyde. Because the luciferase reaction generates light, the enzyme has the potential to be used as a bioreporter for a wide variety of applications. With the recent invention of the fusion enzyme that can be expressed in mammalian cells, future possibilities for the development of additional bioreporter applications are promising. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Russia | 1 | 3% |
Unknown | 33 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 9 | 26% |
Student > Master | 6 | 18% |
Researcher | 3 | 9% |
Student > Ph. D. Student | 3 | 9% |
Student > Doctoral Student | 1 | 3% |
Other | 1 | 3% |
Unknown | 11 | 32% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 10 | 29% |
Agricultural and Biological Sciences | 4 | 12% |
Medicine and Dentistry | 2 | 6% |
Engineering | 2 | 6% |
Chemistry | 2 | 6% |
Other | 3 | 9% |
Unknown | 11 | 32% |