| Chapter title |
Filamentous Phage: Structure and Biology
|
|---|---|
| Chapter number | 1 |
| Book title |
Recombinant Antibodies for Infectious Diseases
|
| Published in |
Advances in experimental medicine and biology, January 2017
|
| DOI | 10.1007/978-3-319-72077-7_1 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-972076-0, 978-3-31-972077-7
|
| Authors |
Rakonjac, Jasna, Russel, Marjorie, Khanum, Sofia, Brooke, Sam J., Rajič, Marina, Jasna Rakonjac, Marjorie Russel, Sofia Khanum, Sam J. Brooke, Marina Rajič |
| Abstract |
Ff filamentous phage (fd, M13 and f1) of Escherichia coli have been the workhorse of phage display technology for the past 30 years. Dominance of Ff over other bacteriophage in display technology stems from the titres that are about 100-fold higher than any other known phage, efficacious transformation ensuring large library size and superior stability of the virion at high temperatures, detergents and pH extremes, allowing broad range of biopanning conditions in screening phage display libraries. Due to the excellent understanding of infection and assembly requirements, Ff phage have also been at the core of phage-assisted continual protein evolution strategies (PACE). This chapter will give an overview of the Ff filamentous phage structure and biology, emphasizing those properties of the Ff phage life cycle and virion that are pertinent to phage display applications. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 113 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 15% |
| Student > Master | 15 | 13% |
| Student > Bachelor | 15 | 13% |
| Researcher | 14 | 12% |
| Student > Doctoral Student | 4 | 4% |
| Other | 13 | 12% |
| Unknown | 35 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 41 | 36% |
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| Chemistry | 7 | 6% |
| Immunology and Microbiology | 4 | 4% |
| Medicine and Dentistry | 3 | 3% |
| Other | 10 | 9% |
| Unknown | 39 | 35% |