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Neurodegeneration

Overview of attention for book
Cover of 'Neurodegeneration'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Introduction to Neurodegenerative Diseases and Related Techniques
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    Chapter 2 In Vivo and In Vitro Determination of Cell Death Markers in Neurons
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    Chapter 3 Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods
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    Chapter 4 Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex
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    Chapter 5 Isolation and Culture of Postnatal Spinal Motoneurons
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    Chapter 6 Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration
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    Chapter 7 Neurodegeneration
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    Chapter 8 Developing yeast models of human neurodegenerative disorders.
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    Chapter 9 Modeling Dopamine Neuron Degeneration in Caenorhabditis elegans
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    Chapter 10 Role of the Proteasome in Fly Models of Neurodegeneration
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    Chapter 11 Neurodegeneration
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    Chapter 12 Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases
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    Chapter 13 Modeling Focal Cerebral Ischemia In Vivo
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    Chapter 14 Pharmacological Models of Parkinson’s Disease in Rodents
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    Chapter 15 Behavioral Phenotyping of Mouse Models of Neurodegeneration
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    Chapter 16 Characterization of Amyloid Deposits in Neurodegenerative Diseases
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    Chapter 17 Detection of Protein Aggregation in Neurodegenerative Diseases
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    Chapter 18 Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures
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    Chapter 19 Measurements of Threshold of Mitochondrial Permeability Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium
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    Chapter 20 Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue
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    Chapter 21 Monitoring Mitophagy in Neuronal Cell Cultures
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    Chapter 22 Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging
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    Chapter 23 Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices
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    Chapter 24 Imaging Presynaptic Exocytosis in Corticostriatal Slices
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    Chapter 25 Microscopic Imaging of Intracellular Calcium in Live Cells Using Lifetime-Based Ratiometric Measurements of Oregon Green BAPTA-1
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    Chapter 26 Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice
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    Chapter 27 Determination of Neurotransmitter Levels in Models of Parkinson’s Disease by HPLC-ECD
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    Chapter 28 Designing, Performing, and Interpreting a Microarray-Based Gene Expression Study
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    Chapter 29 Adeno-Associated Viral Gene Delivery in Neurodegenerative Disease
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    Chapter 30 Specific Gene Silencing Using RNAi in Cell Culture.
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    Chapter 31 Stem Cell Transplantation for Spinal Cord Neurodegeneration
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    Chapter 32 Evaluation of histone deacetylase inhibitors as therapeutics for neurodegenerative diseases.
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    Chapter 33 Erratum to: Microscopic Imaging of Intracellular Calcium in Live Cells Using Lifetime-Based Ratiometric Measurements of Oregon Green BAPTA-1
  35. Altmetric Badge
    Chapter 34 Erratum to: Adeno-Associated Viral Gene Delivery in Neurodegenerative Disease
Attention for Chapter 30: Specific Gene Silencing Using RNAi in Cell Culture.
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

Mentioned by

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1 news outlet
twitter
3 tweeters

Citations

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23 Dimensions

Readers on

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82 Mendeley
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Chapter title
Specific Gene Silencing Using RNAi in Cell Culture.
Chapter number 30
Book title
Neurodegeneration
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-328-8_30
Pubmed ID
Book ISBNs
978-1-61779-327-1, 978-1-61779-328-8
Authors

Chunxing Yang, Linghua Qiu, Zuoshang Xu, Yang, Chunxing, Qiu, Linghua, Xu, Zuoshang

Abstract

RNA interference (RNAi) is a conserved cellular mechanism in most eukaryotes that can mediate specific gene silencing. Since its discovery in 1998, rapid progress has been made in understanding its basic mechanism and its application in research and drug discovery. In recent years, the application of RNAi in research, including research in neurodegeneration, has expanded rapidly such that it has become a regular tool for reverse genetics in cultured cells in many labs. However, an incomplete understanding of the RNAi mechanism and worries about its pitfalls still intimidate many others. Here, we present a streamlined and simple protocol for the design and implementation of an RNAi experiment in cultured cells, aiming to enable those who are inexperienced with RNAi to apply this powerful method in their research, particularly in the field of neurodegeneration.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 82 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 82 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 21 26%
Student > Bachelor 20 24%
Student > Master 10 12%
Researcher 7 9%
Student > Postgraduate 3 4%
Other 7 9%
Unknown 14 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 38 46%
Agricultural and Biological Sciences 11 13%
Medicine and Dentistry 5 6%
Pharmacology, Toxicology and Pharmaceutical Science 3 4%
Neuroscience 3 4%
Other 9 11%
Unknown 13 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 August 2020.
All research outputs
#2,796,557
of 22,651,245 outputs
Outputs from Methods in molecular biology
#542
of 13,012 outputs
Outputs of similar age
#17,329
of 180,239 outputs
Outputs of similar age from Methods in molecular biology
#14
of 229 outputs
Altmetric has tracked 22,651,245 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,012 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 180,239 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 229 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.