Report for: Neurodegeneration

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Chapter title	Specific Gene Silencing Using RNAi in Cell Culture.
Chapter number	30
Book title	Neurodegeneration
Published in	Methods in molecular biology, January 2011
DOI	10.1007/978-1-61779-328-8_30
Pubmed ID	21913119
Book ISBNs	978-1-61779-327-1, 978-1-61779-328-8
Authors	Chunxing Yang, Linghua Qiu, Zuoshang Xu, Yang, Chunxing, Qiu, Linghua, Xu, Zuoshang
Abstract	RNA interference (RNAi) is a conserved cellular mechanism in most eukaryotes that can mediate specific gene silencing. Since its discovery in 1998, rapid progress has been made in understanding its basic mechanism and its application in research and drug discovery. In recent years, the application of RNAi in research, including research in neurodegeneration, has expanded rapidly such that it has become a regular tool for reverse genetics in cultured cells in many labs. However, an incomplete understanding of the RNAi mechanism and worries about its pitfalls still intimidate many others. Here, we present a streamlined and simple protocol for the design and implementation of an RNAi experiment in cultured cells, aiming to enable those who are inexperienced with RNAi to apply this powerful method in their research, particularly in the field of neurodegeneration.

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Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Geographical breakdown

Country	Count	As %
Australia	1	33%
Turkey	1	33%
United States	1	33%

Demographic breakdown

Type	Count	As %
Scientists	2	67%
Members of the public	1	33%

Mendeley readers

The data shown below were compiled from readership statistics for 84 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Unknown	84	100%

Demographic breakdown

Readers by professional status	Count	As %
Student > Ph. D. Student	21	25%
Student > Bachelor	20	24%
Student > Master	10	12%
Researcher	7	8%
Student > Postgraduate	3	4%
Other	7	8%
Unknown	16	19%

Readers by discipline	Count	As %
Biochemistry, Genetics and Molecular Biology	39	46%
Agricultural and Biological Sciences	11	13%
Medicine and Dentistry	5	6%
Pharmacology, Toxicology and Pharmaceutical Science	3	4%
Neuroscience	3	4%
Other	8	10%
Unknown	15	18%

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 August 2020.

All research outputs

#2,796,557

of 22,651,245 outputs

Outputs from Methods in molecular biology

#542

of 13,012 outputs

Outputs of similar age

#17,329

of 180,239 outputs

Outputs of similar age from Methods in molecular biology

#14

of 229 outputs

Altmetric has tracked 22,651,245 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.

So far Altmetric has tracked 13,012 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done particularly well, scoring higher than 95% of its peers.

Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 180,239 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.

We're also able to compare this research output to 229 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.

Neurodegeneration

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Geographical breakdown

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Attention Score in Context