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Mendeley readers
| Chapter title |
Determination of Acetylation of the Gli Transcription Factors
|
|---|---|
| Chapter number | 13 |
| Book title |
Hedgehog Signaling Protocols
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2772-2_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2771-5, 978-1-4939-2772-2
|
| Authors |
Sonia Coni, Laura Di Magno, Gianluca Canettieri |
| Abstract |
The Gli transcription factors (Gli1, Gli2, and Gli3) are the final effectors of the Hedgehog (Hh) signaling and play a key role in development and cancer. The activity of the Gli proteins is finely regulated by covalent modifications, such as phosphorylation, ubiquitination, and acetylation. Both Gli1 and Gli2 are acetylated at a conserved lysine, and this modification causes the inhibition of their transcriptional activity. Thus, the acetylation status of these proteins represents a useful marker to monitor Hh activation in pathophysiological conditions. Herein we describe the techniques utilized to detect in vitro and intracellular acetylation of the Gli transcription factors. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 1 | 50% |
| Student > Master | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Mathematics | 1 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 50% |