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Mendeley readers
Chapter title |
Rapid Screening of Gli2/3 Mutants Using the Flp-In System
|
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Chapter number | 11 |
Book title |
Hedgehog Signaling Protocols
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2772-2_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2771-5, 978-1-4939-2772-2
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Authors |
Pawel Niewiadomski, Rajat Rohatgi |
Abstract |
Gli2 and Gli3 respond to the Hedgehog (Hh) signal in mammals by undergoing posttranslational modifications and moving to the nucleus. The study of Gli proteins has been hampered by the fact that their overexpression in cells prevents their proper regulation. To address this issue, we have developed a method of rapid generation of stable cell lines expressing near-endogenous and approximately equal levels of wild-type and mutant Gli proteins. This method is applicable to the study of effects of various mutations on Gli protein modifications and activity. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 1 | 33% |
Student > Bachelor | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Agricultural and Biological Sciences | 1 | 33% |
Unknown | 1 | 33% |