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Mendeley readers
| Chapter title |
Luciferase Reporter Assays to Study Transcriptional Activity of Hedgehog Signaling in Normal and Cancer Cells
|
|---|---|
| Chapter number | 7 |
| Book title |
Hedgehog Signaling Protocols
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2772-2_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2771-5, 978-1-4939-2772-2
|
| Authors |
Silvia Pandolfi, Barbara Stecca |
| Abstract |
The measurement of the transcriptional activity of the HH signaling pathway is widely used as an indication of pathway activation. Luciferase reporter assays are powerful tools to measure the specific ability of a transcription factor to bind to its consensus sequence and to activate transcription of target genes. Here, we describe a protocol to measure the transcriptional activity of the HH pathway in normal and cancer cells. This technique allows studying the activity of GLI transcription factors and their modulation by drugs and/or other factors. |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 26% |
| Student > Ph. D. Student | 5 | 19% |
| Researcher | 4 | 15% |
| Student > Postgraduate | 3 | 11% |
| Student > Bachelor | 3 | 11% |
| Other | 1 | 4% |
| Unknown | 4 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 15 | 56% |
| Medicine and Dentistry | 4 | 15% |
| Chemistry | 2 | 7% |
| Agricultural and Biological Sciences | 2 | 7% |
| Unknown | 4 | 15% |