Chapter title |
Heterogeneity of ANCA sera showing atypical, peripheral and classical cytoplasmic immunofluorescence patterns.
|
---|---|
Chapter number | 35 |
Book title |
ANCA-Associated Vasculitides
|
Published in |
Advances in experimental medicine and biology, January 1993
|
DOI | 10.1007/978-1-4757-9182-2_35 |
Pubmed ID | |
Book ISBNs |
978-1-4757-9184-6, 978-1-4757-9182-2
|
Authors |
Dalpé, G, Fernandes, F, Richard, C, Boire, G, Ménard, H A, Gratien Dalpé, Fatima Fernandes, Carol Richard, Gilles Boire, Henri A. Ménard, Dalpé, Gratien, Fernandes, Fatima, Richard, Carol, Boire, Gilles, Ménard, Henri A. |
Abstract |
Atypical (A) ANCA immunofluorescence (IF) patterns have been described in several disease groups. We have previously reported a distinct cytoplasmic A-ANCA in 7-10% of patients with SLE and/or RA. Here, we show that these rheumatic disease associated A-ANCAs are best identified using U937 cells as substrate and that they do not target either a serine proteinase or a peroxidase. Furthermore, these sera immunoprecipitate a 40 kDa or a 42 kDa band using in vivo 35S-amino acid labelled HL60 or U937 cell extracts, respectively. Although these bands are the only one seen with pure A-ANCA sera, they can also be found in addition to the expected bands of PR3 or MPO in up to 30% of bona fide C- or P-ANCA sera. These data confirm and extend our previous observations. They also suggest that target heterogeneity of ANCA antibodies is frequent. Care should thus be taken in interpreting in a cause and effect relationship, an IF pattern or a biological effect produced by a serum with ill documented monospecificity. The exact nature and significance of this (these) new antigen(s) have yet to be clarified. |
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