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Patch-Clamp Methods and Protocols

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Cover of 'Patch-Clamp Methods and Protocols'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 History of Electrophysiology and the Patch Clamp
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    Chapter 2 Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications
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    Chapter 3 Patch Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N -Methyl- d -aspartate (NMDA) Receptor Open Channel Blockers
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    Chapter 4 State-of-the-Art Automated Patch Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
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    Chapter 5 Industrializing Electrophysiology: HT Automated Patch Clamp on SyncroPatch ® 96 Using Instant Frozen Cells
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    Chapter 6 Planar Patch Clamp for Neuronal Networks—Considerations and Future Perspectives
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    Chapter 7 Methods for the Study of Synaptic Receptor Functional Properties
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    Chapter 8 Single-Cell RT-PCR, a Technique to Decipher the Electrical, Anatomical, and Genetic Determinants of Neuronal Diversity
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    Chapter 9 Whole-Cell Voltage Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique
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    Chapter 10 Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability
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    Chapter 11 Investigation of Synaptic Microcircuits Using Patch-Clamp Paired Recordings in Acute Brain Slices
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    Chapter 12 Outside-Out “Sniffer-Patch” Clamp Technique for In Situ Measures of Neurotransmitter Release
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    Chapter 13 A Cost-Effective Method for Preparing, Maintaining, and Transfecting Neurons in Organotypic Slices
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    Chapter 14 Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics
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    Chapter 15 Synaptic Connectivity in Engineered Neuronal Networks
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    Chapter 16 Modeling of Action Potential Generation in NG108-15 Cells
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    Chapter 17 Whole-Cell Patch-Clamp Recordings in Freely Moving Animals
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    Chapter 18 Pressure-Polished Borosilicate Pipettes are “Universal Sealer” Yielding Low Access Resistance and Efficient Intracellular Perfusion
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    Chapter 19 Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes
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    Chapter 20 Patch-Clamp Methods and Protocols
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    Chapter 21 Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI
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    Chapter 22 Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes
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    Chapter 23 Principles of Single-Channel Kinetic Analysis
Attention for Chapter 14: Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

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Chapter title
Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics
Chapter number 14
Book title
Patch-Clamp Methods and Protocols
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-1096-0_14
Pubmed ID
Book ISBNs
978-1-4939-1095-3, 978-1-4939-1096-0
Authors

Jonathan T. Ting, Tanya L. Daigle, Qian Chen, Guoping Feng, Ting, Jonathan T., Daigle, Tanya L., Chen, Qian, Feng, Guoping

Abstract

The development of the living acute brain slice preparation for analyzing synaptic function roughly a half century ago was a pivotal achievement that greatly influenced the landscape of modern neuroscience. Indeed, many neuroscientists regard brain slices as the gold-standard model system for detailed cellular, molecular, and circuitry level analysis and perturbation of neuronal function. A critical limitation of this model system is the difficulty in preparing slices from adult and aging animals, and over the past several decades few substantial methodological improvements have emerged to facilitate patch clamp analysis in the mature adult stage. In this chapter we describe a robust and practical protocol for preparing brain slices from mature adult mice that are suitable for patch clamp analysis. This method reduces swelling and damage in superficial layers of the slices and improves the success rate for targeted patch clamp recordings, including recordings from fluorescently labeled populations in slices derived from transgenic mice. This adult brain slice method is suitable for diverse experimental applications, including both monitoring and manipulating neuronal activity with genetically encoded calcium indicators and optogenetic actuators, respectively. We describe the application of this adult brain slice platform and associated methods for screening kinetic properties of Channelrhodopsin (ChR) variants expressed in genetically defined neuronal subtypes.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 561 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 5 <1%
United Kingdom 3 <1%
France 2 <1%
Hungary 1 <1%
Australia 1 <1%
Germany 1 <1%
Netherlands 1 <1%
Romania 1 <1%
Canada 1 <1%
Other 2 <1%
Unknown 543 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 144 26%
Researcher 96 17%
Student > Master 51 9%
Student > Bachelor 49 9%
Student > Doctoral Student 35 6%
Other 72 13%
Unknown 114 20%
Readers by discipline Count As %
Neuroscience 210 37%
Agricultural and Biological Sciences 119 21%
Biochemistry, Genetics and Molecular Biology 31 6%
Medicine and Dentistry 19 3%
Engineering 17 3%
Other 37 7%
Unknown 128 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 October 2022.
All research outputs
#3,748,223
of 23,523,017 outputs
Outputs from Methods in molecular biology
#922
of 13,389 outputs
Outputs of similar age
#43,970
of 308,923 outputs
Outputs of similar age from Methods in molecular biology
#37
of 587 outputs
Altmetric has tracked 23,523,017 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,389 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 308,923 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 587 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.