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Patch-Clamp Methods and Protocols

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Cover of 'Patch-Clamp Methods and Protocols'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 History of Electrophysiology and the Patch Clamp
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    Chapter 2 Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications
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    Chapter 3 Patch Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N -Methyl- d -aspartate (NMDA) Receptor Open Channel Blockers
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    Chapter 4 State-of-the-Art Automated Patch Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
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    Chapter 5 Industrializing Electrophysiology: HT Automated Patch Clamp on SyncroPatch ® 96 Using Instant Frozen Cells
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    Chapter 6 Planar Patch Clamp for Neuronal Networks—Considerations and Future Perspectives
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    Chapter 7 Methods for the Study of Synaptic Receptor Functional Properties
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    Chapter 8 Single-Cell RT-PCR, a Technique to Decipher the Electrical, Anatomical, and Genetic Determinants of Neuronal Diversity
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    Chapter 9 Whole-Cell Voltage Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique
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    Chapter 10 Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability
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    Chapter 11 Investigation of Synaptic Microcircuits Using Patch-Clamp Paired Recordings in Acute Brain Slices
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    Chapter 12 Outside-Out “Sniffer-Patch” Clamp Technique for In Situ Measures of Neurotransmitter Release
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    Chapter 13 A Cost-Effective Method for Preparing, Maintaining, and Transfecting Neurons in Organotypic Slices
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    Chapter 14 Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics
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    Chapter 15 Synaptic Connectivity in Engineered Neuronal Networks
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    Chapter 16 Modeling of Action Potential Generation in NG108-15 Cells
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    Chapter 17 Whole-Cell Patch-Clamp Recordings in Freely Moving Animals
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    Chapter 18 Pressure-Polished Borosilicate Pipettes are “Universal Sealer” Yielding Low Access Resistance and Efficient Intracellular Perfusion
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    Chapter 19 Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes
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    Chapter 20 Patch-Clamp Methods and Protocols
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    Chapter 21 Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI
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    Chapter 22 Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes
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    Chapter 23 Principles of Single-Channel Kinetic Analysis
Attention for Chapter 1: History of Electrophysiology and the Patch Clamp
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

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2 Wikipedia pages

Citations

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Chapter title
History of Electrophysiology and the Patch Clamp
Chapter number 1
Book title
Patch-Clamp Methods and Protocols
Published in
Methods in molecular biology, June 2014
DOI 10.1007/978-1-4939-1096-0_1
Pubmed ID
Book ISBNs
978-1-4939-1095-3, 978-1-4939-1096-0
Authors

Verkhratsky A, Parpura V, Alexei Verkhratsky, Vladimir Parpura, Verkhratsky, Alexei, Parpura, Vladimir

Abstract

We provide a historic outlook on the development of the concept of bioelectricity, with emphasis on the neuromuscular junction as a model that revolutionized our thinking of the nerve, nervous, and muscle tissue excitability. We abridge some crucial experiments in defining the electrical excitability of biological cells. We also provide an insight into developments of tools and methods, which gradually yielded a contemporary "palette" of electrophysiology approaches, including the patch clamp. Pioneering steps in this journey, ranging from Galvani's experiments using the Leyden jar to those of Neher and Sakmann using a gigaseal patch-clamp approach, are pictorially illustrated. This chapter is meant to be a perspective to the following sections in this volume dedicated to patch-clamp methods and protocols.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 220 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 <1%
Portugal 1 <1%
Brazil 1 <1%
Unknown 217 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 45 20%
Researcher 40 18%
Student > Master 26 12%
Student > Bachelor 24 11%
Student > Doctoral Student 17 8%
Other 29 13%
Unknown 39 18%
Readers by discipline Count As %
Neuroscience 54 25%
Agricultural and Biological Sciences 47 21%
Biochemistry, Genetics and Molecular Biology 22 10%
Engineering 12 5%
Physics and Astronomy 10 5%
Other 28 13%
Unknown 47 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 March 2023.
All research outputs
#2,283,322
of 23,567,572 outputs
Outputs from Methods in molecular biology
#388
of 13,353 outputs
Outputs of similar age
#23,620
of 229,702 outputs
Outputs of similar age from Methods in molecular biology
#1
of 108 outputs
Altmetric has tracked 23,567,572 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,353 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 229,702 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 108 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.