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Patch-Clamp Methods and Protocols

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Cover of 'Patch-Clamp Methods and Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 History of Electrophysiology and the Patch Clamp
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    Chapter 2 Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications
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    Chapter 3 Patch Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N -Methyl- d -aspartate (NMDA) Receptor Open Channel Blockers
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    Chapter 4 State-of-the-Art Automated Patch Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
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    Chapter 5 Industrializing Electrophysiology: HT Automated Patch Clamp on SyncroPatch ® 96 Using Instant Frozen Cells
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    Chapter 6 Planar Patch Clamp for Neuronal Networks—Considerations and Future Perspectives
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    Chapter 7 Methods for the Study of Synaptic Receptor Functional Properties
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    Chapter 8 Single-Cell RT-PCR, a Technique to Decipher the Electrical, Anatomical, and Genetic Determinants of Neuronal Diversity
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    Chapter 9 Whole-Cell Voltage Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique
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    Chapter 10 Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability
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    Chapter 11 Investigation of Synaptic Microcircuits Using Patch-Clamp Paired Recordings in Acute Brain Slices
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    Chapter 12 Outside-Out “Sniffer-Patch” Clamp Technique for In Situ Measures of Neurotransmitter Release
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    Chapter 13 A Cost-Effective Method for Preparing, Maintaining, and Transfecting Neurons in Organotypic Slices
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    Chapter 14 Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics
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    Chapter 15 Synaptic Connectivity in Engineered Neuronal Networks
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    Chapter 16 Modeling of Action Potential Generation in NG108-15 Cells
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    Chapter 17 Whole-Cell Patch-Clamp Recordings in Freely Moving Animals
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    Chapter 18 Pressure-Polished Borosilicate Pipettes are “Universal Sealer” Yielding Low Access Resistance and Efficient Intracellular Perfusion
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    Chapter 19 Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes
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    Chapter 20 Patch-Clamp Methods and Protocols
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    Chapter 21 Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI
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    Chapter 22 Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes
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    Chapter 23 Principles of Single-Channel Kinetic Analysis
Attention for Chapter 8: Single-Cell RT-PCR, a Technique to Decipher the Electrical, Anatomical, and Genetic Determinants of Neuronal Diversity
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Chapter title
Single-Cell RT-PCR, a Technique to Decipher the Electrical, Anatomical, and Genetic Determinants of Neuronal Diversity
Chapter number 8
Book title
Patch-Clamp Methods and Protocols
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-1096-0_8
Pubmed ID
Book ISBNs
978-1-4939-1095-3, 978-1-4939-1096-0
Authors

Maria Toledo-Rodriguez, Henry Markram, Toledo-Rodriguez, Maria, Markram, Henry

Abstract

The patch-clamp technique has allowed for detailed studies on the electrical properties of neurons. Dye loading through patch pipettes enabled characterizing the morphological properties of the neurons. In addition, the patch-clamp technique also allows for harvesting mRNA from single cells to study gene expression at the single cell level (known as single-cell RT-PCR). The combination of these three approaches makes possible the study of the GEM profile of neurons (gene expression, electrophysiology, and morphology) using a single patch pipette and patch-clamp recording. This combination provides a powerful technique to investigate and correlate the neuron's gene expression with its phenotype (electrical behavior and morphology). The harvesting and amplification of single cell mRNA for gene expression studies is a challenging task, especially for researchers with sparse or no training in molecular biology (see Notes 1,2 and 5). Here we describe in detail the GEM profiling approach with special attention to the gene expression profiling.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 3%
Unknown 37 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 24%
Student > Bachelor 7 18%
Researcher 6 16%
Student > Master 3 8%
Other 2 5%
Other 5 13%
Unknown 6 16%
Readers by discipline Count As %
Neuroscience 14 37%
Agricultural and Biological Sciences 10 26%
Physics and Astronomy 3 8%
Engineering 2 5%
Unspecified 1 3%
Other 2 5%
Unknown 6 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 August 2014.
All research outputs
#18,376,056
of 22,760,687 outputs
Outputs from Methods in molecular biology
#7,870
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Outputs of similar age
#229,413
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Outputs of similar age from Methods in molecular biology
#294
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