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Mendeley readers
Chapter title |
Cytoplasmic polyadenylation assays.
|
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Chapter number | 5 |
Book title |
Polyadenylation
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Published in |
Methods in molecular biology, March 2014
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DOI | 10.1007/978-1-62703-971-0_5 |
Pubmed ID | |
Book ISBNs |
978-1-62703-970-3, 978-1-62703-971-0
|
Authors |
Coll O, Villalba A, Gebauer F, Olga Coll, Ana Villalba, Fátima Gebauer |
Abstract |
Basic research in Drosophila melanogaster has benefited from a plethora of powerful genetics tools. Detailed biochemical analysis, however, has often been difficult due to the lack of in vitro systems that faithfully recapitulate the observations made in vivo. In the field of posttranscriptional regulation, the recent establishment of robust in vitro systems from embryo and ovary material has fueled the mechanistic understanding of a variety of processes. Here we describe protocols to obtain and use extracts from Drosophila embryos that are competent for cytoplasmic polyadenylation and translation of exogenously added transcripts. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 50% |
Professor | 1 | 17% |
Professor > Associate Professor | 1 | 17% |
Researcher | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 83% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |