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Mendeley readers
Chapter title |
Assaying mRNA deadenylation in vitro.
|
---|---|
Chapter number | 24 |
Book title |
Polyadenylation
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Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-62703-971-0_24 |
Pubmed ID | |
Book ISBNs |
978-1-62703-970-3, 978-1-62703-971-0
|
Authors |
Mandy Jeske, Claudia Temme, Elmar Wahle, Jeske, Mandy, Temme, Claudia, Wahle, Elmar |
Abstract |
Deadenylation is the removal of poly(A) tails from mRNA. This chapter presents two methods to assay deadenylation in vitro. The first is a quick and quantitative assay for the degradation of radiolabeled poly(A) that can easily be adapted to be used for many different enzymes. The second method uses an extract from Drosophila embryos to catalyze the deadenylation of an RNA dependent on a specific sequence that also directs deadenylation in vivo. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 25% |
Student > Bachelor | 1 | 13% |
Other | 1 | 13% |
Student > Ph. D. Student | 1 | 13% |
Researcher | 1 | 13% |
Other | 0 | 0% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 50% |
Agricultural and Biological Sciences | 2 | 25% |
Unknown | 2 | 25% |