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Polyadenylation

Overview of attention for book
Cover of 'Polyadenylation'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 3′ End Formation and Regulation of Eukaryotic mRNAs
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    Chapter 2 Retracted: Poly(A) Polymerase-Based Poly(A) Length Assay
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    Chapter 3 Using Klenow-Mediated Extension to Measure Poly(A)-Tail Length and Position in the Transcriptome
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    Chapter 4 Detection of Uridylated mRNAs
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    Chapter 5 Cytoplasmic polyadenylation assays.
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    Chapter 6 In Vitro Analysis of Transcriptional Activators and Polyadenylation
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    Chapter 7 Fluorescence Detection of Polyadenylation Reaction Through the Coordination of Adenosine 2 –Coralyne–Adenosine 2
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    Chapter 8 Small Molecule Recognition of Poly(A)
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    Chapter 9 The Stem-Loop Luciferase Assay for Polyadenylation (SLAP) Method for Determining CstF-64-Dependent Polyadenylation Activity
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    Chapter 10 Accurate Mapping of Cleavage and Polyadenylation Sites by 3′ Region Extraction and Deep Sequencing
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    Chapter 11 Polyadenylation Site Prediction Using PolyA-iEP Method
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    Chapter 12 Detecting Alternative Polyadenylation from Microarray Data
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    Chapter 13 Quantitative Polyadenylation Site Mapping with Single-Molecule Direct RNA Sequencing
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    Chapter 14 RHAPA: A New Method to Quantify Alternative Polyadenylation
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    Chapter 15 Multiplex Analysis of PolyA-Linked Sequences (MAPS): An RNA-Seq Strategy to Profile Poly(A+) RNA
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    Chapter 16 Global and Quantitative Profiling of Polyadenylated RNAs Using PAS-seq.
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    Chapter 17 High-Throughput Sequencing of RNA Isolated by Cross-Linking and Immunoprecipitation (HITS-CLIP) to Determine Sites of Binding of CstF-64 on Nascent RNAs
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    Chapter 18 Polyadenylation in Bacteria and Organelles
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    Chapter 19 In Vivo Analysis of Polyadenylation in Prokaryotes
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    Chapter 20 Polyadenylation of RNA in E. coli : RNA Polymerase-Associated (rA) n -Synthetic Activities
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    Chapter 21 Analysis of the human mitochondrial transcriptome using directional deep sequencing and parallel analysis of RNA ends.
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    Chapter 22 Measurement of Mitochondrial RNA Stability by Metabolic Labeling of Transcripts with 4-Thiouridine
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    Chapter 23 Deadenylation and Its Regulation in Eukaryotic Cells
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    Chapter 24 Assaying mRNA deadenylation in vitro.
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    Chapter 25 Assaying mRNA deadenylation in vivo.
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    Chapter 26 In Vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes
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    Chapter 27 MicroRNA-Mediated Deadenylation in a Mammalian Cell-Free System
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    Chapter 28 Quantitative Analysis of Deadenylation-Independent mRNA Decay by a Modified MBRACE Assay
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    Chapter 29 Erratum To: Cytoplasmic Polyadenylation Assays
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    Chapter 30 Erratum: Retracted: Poly(A) Polymerase-Based Poly(A) Length Assay
Attention for Chapter 16: Global and Quantitative Profiling of Polyadenylated RNAs Using PAS-seq.
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Chapter title
Global and Quantitative Profiling of Polyadenylated RNAs Using PAS-seq.
Chapter number 16
Book title
Polyadenylation
Published in
Methods in molecular biology, March 2014
DOI 10.1007/978-1-62703-971-0_16
Pubmed ID
Book ISBNs
978-1-62703-970-3, 978-1-62703-971-0
Authors

Yao C, Shi Y, Chengguo Yao, Yongsheng Shi

Abstract

mRNA alternative polyadenylation (APA) has been increasingly recognized as a widespread and evolutionarily conserved mechanism for eukaryotic gene regulation. Here we describe a method called poly(A) site sequencing that can not only map RNA polyadenylation sites on a transcriptome level but also provide quantitative information on the relative abundance of polyadenylated RNAs. This method has been successfully used for both global APA analysis and digital gene expression profiling.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 27%
Professor > Associate Professor 3 20%
Researcher 3 20%
Student > Bachelor 1 7%
Student > Doctoral Student 1 7%
Other 1 7%
Unknown 2 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 47%
Biochemistry, Genetics and Molecular Biology 3 20%
Computer Science 2 13%
Unknown 3 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2014.
All research outputs
#18,369,403
of 22,751,628 outputs
Outputs from Methods in molecular biology
#7,861
of 13,088 outputs
Outputs of similar age
#160,973
of 221,377 outputs
Outputs of similar age from Methods in molecular biology
#48
of 146 outputs
Altmetric has tracked 22,751,628 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,088 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 146 others from the same source and published within six weeks on either side of this one. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.