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Prions

Overview of attention for book
Cover of 'Prions'

Table of Contents

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    Book Overview
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    Chapter 1 Purification and Fibrillation of Full-Length Recombinant PrP
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    Chapter 2 Method for Folding of Recombinant Prion Protein to Soluble β-Sheet Secondary Structure
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    Chapter 3 Analysis of Prion Protein Conformation Using Circular Dichroism Spectroscopy
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    Chapter 4 Analysis of Prion Protein Structure Using Nuclear Magnetic Resonance Spectroscopy
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    Chapter 5 Immunodetection of PrP Sc Using Western Immunoblotting Techniques
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    Chapter 6 Analysis of miRNA Signatures in Neurodegenerative Prion Disease
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    Chapter 7 Cell Biology Approaches to Studying Prion Diseases
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    Chapter 8 Expression of Heterologous PrP and Prion Propagation in RK13 Cells
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    Chapter 9 Generation of Infectious Prions and Detection with the Prion-Infected Cell Assay
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    Chapter 10 Analysis of Cellular Prion Protein Endoproteolytic Processing
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    Chapter 11 Cellular Analysis of Adult Neural Stem Cells for Investigating Prion Biology
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    Chapter 12 Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons
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    Chapter 13 Methods of Protein Misfolding Cyclic Amplification
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    Chapter 14 RT-QuIC Assays for Prion Disease Detection and Diagnostics
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    Chapter 15 A Quick Method to Evaluate the Effect of the Amino Acid Sequence in the Misfolding Proneness of the Prion Protein
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    Chapter 16 Insights into Mechanisms of Transmission and Pathogenesis from Transgenic Mouse Models of Prion Diseases
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    Chapter 17 In Vivo-Near Infrared Imaging of Neurodegeneration
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    Chapter 18 Strain Typing of Prion Diseases Using In Vivo Mouse Models
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    Chapter 19 Preparation and Immunostaining of the Myenteric Plexus of Prion-Infected Mice
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    Chapter 20 Cell Culture Methods for Screening of Prion Therapeutics
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    Chapter 21 Real-Time Quaking-Induced Conversion for Diagnosis of Prion Disease
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    Chapter 22 Methods for Molecular Diagnosis of Human Prion Disease
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    Chapter 23 Molecular Subtyping of PrPres in Human Sporadic CJD Brain Tissue
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    Chapter 24 Intercellular Prion-Like Conversion and Transmission of Cu/Zn Superoxide Dismutase (SOD1) in Cell Culture
Attention for Chapter 22: Methods for Molecular Diagnosis of Human Prion Disease
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Chapter title
Methods for Molecular Diagnosis of Human Prion Disease
Chapter number 22
Book title
Prions
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7244-9_22
Pubmed ID
28861799
Book ISBNs
978-1-4939-7242-5, 978-1-4939-7244-9
Authors

Jonathan D. F. Wadsworth, Gary Adamson, Susan Joiner, Lara Brock, Caroline Powell, Jacqueline M. Linehan, Jonathan A. Beck, Sebastian Brandner, Simon Mead, John Collinge, Wadsworth, JDF, Adamson, G, Joiner, S, Brock, L, Powell, C, Linehan, JM, Beck, JA, Brandner, S, Mead, S, Collinge, J, Wadsworth, Jonathan D. F., Adamson, Gary, Joiner, Susan, Brock, Lara, Powell, Caroline, Linehan, Jacqueline M., Beck, Jonathan A., Brandner, Sebastian, Mead, Simon, Collinge, John

Abstract

Human prion diseases are associated with a range of clinical presentations, and they are classified by both clinicopathological syndrome and etiology, with subclassification according to molecular criteria. Here, we describe updated procedures that are currently used within the MRC Prion Unit at UCL to determine a molecular diagnosis of human prion disease. Sequencing of the PRNP open reading frame to establish the presence of pathogenic mutations is described, together with detailed methods for immunoblot or immunohistochemical determination of the presence of abnormal prion protein in the brain or peripheral tissues.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 21%
Student > Master 3 13%
Professor 2 8%
Student > Ph. D. Student 2 8%
Student > Postgraduate 2 8%
Other 2 8%
Unknown 8 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 25%
Agricultural and Biological Sciences 3 13%
Neuroscience 2 8%
Medicine and Dentistry 2 8%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Other 1 4%
Unknown 9 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 September 2018.
All research outputs
#20,481,952
of 23,043,346 outputs
Outputs from Methods in molecular biology
#9,975
of 13,194 outputs
Outputs of similar age
#356,334
of 421,409 outputs
Outputs of similar age from Methods in molecular biology
#842
of 1,074 outputs
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So far Altmetric has tracked 13,194 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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