Chapter title |
Methods of Protein Misfolding Cyclic Amplification
|
---|---|
Chapter number | 13 |
Book title |
Prions
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7244-9_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7242-5, 978-1-4939-7244-9
|
Authors |
Natallia Makarava, Regina Savtchenko, Ilia V. Baskakov, Makarava, Natallia, Savtchenko, Regina, Baskakov, Ilia V. |
Abstract |
Protein misfolding cyclic amplification (PMCA) amplifies infectious prions in vitro. Over the past decade, PMCA has become an essential tool in prion research. The current chapter describes in detail the PMCA format with beads (PMCAb) and several methods that rely on PMCAb for assessing strain-specific prion amplification rates, for selective amplification of subtypes of PrP(Sc) from a mixture, and a PMCAb approach that can replace animal titration of scrapie material. Development of PMCAb-based methodology is important for addressing a number of research topics including prion strain evolution, selection and adaptation, strain-typing, prion detection, and biochemical requirements for prion replication. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 26% |
Researcher | 2 | 11% |
Other | 1 | 5% |
Professor | 1 | 5% |
Student > Bachelor | 1 | 5% |
Other | 2 | 11% |
Unknown | 7 | 37% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 21% |
Neuroscience | 3 | 16% |
Agricultural and Biological Sciences | 2 | 11% |
Immunology and Microbiology | 1 | 5% |
Chemistry | 1 | 5% |
Other | 0 | 0% |
Unknown | 8 | 42% |