| Chapter title |
Assessing Sertoli Cell Metabolic Activity
|
|---|---|
| Chapter number | 12 |
| Book title |
Sertoli Cells
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7698-0_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7697-3, 978-1-4939-7698-0
|
| Authors |
Ivana Jarak, Pedro F. Oliveira, Gustavo Rindone, Rui A. Carvalho, María N. Galardo, María F. Riera, Silvina B. Meroni, Marco G. Alves, Jarak, Ivana, Oliveira, Pedro F., Rindone, Gustavo, Carvalho, Rui A., Galardo, María N., Riera, María F., Meroni, Silvina B., Alves, Marco G. |
| Abstract |
Nuclear magnetic resonance (NMR)-based metabolomics is widely used in the research of metabolic conditions of complex biological systems under various conditions, and its use has been found in the field of male fertility. Here we describe the implementation of total and targeted NMR-based metabolomics in the research on Sertoli cell metabolism. Main principles and techniques of cell medium, cellular extracts, and intact cells are explained, as well as some classical experiments that can give complementary information on the Sertoli cell metabolism. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 40% |
| Student > Ph. D. Student | 3 | 30% |
| Student > Bachelor | 1 | 10% |
| Student > Doctoral Student | 1 | 10% |
| Professor | 1 | 10% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 30% |
| Veterinary Science and Veterinary Medicine | 1 | 10% |
| Agricultural and Biological Sciences | 1 | 10% |
| Medicine and Dentistry | 1 | 10% |
| Unknown | 4 | 40% |