Chapter title |
A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells
|
---|---|
Chapter number | 19 |
Book title |
Sertoli Cells
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7698-0_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7697-3, 978-1-4939-7698-0
|
Authors |
Anna Maggio, Raquel L. Bernardino, Patrizia Gena, Marco G. Alves, Pedro F. Oliveira, Giuseppe Calamita, Maggio, Anna, Bernardino, Raquel L., Gena, Patrizia, Alves, Marco G., Oliveira, Pedro F., Calamita, Giuseppe |
Abstract |
Movement of water into and out of the cell is fundamental to life. In the male reproductive tract, at the level of the seminiferous epithelium, Sertoli cells are pivotal in mediating the movement of water into the luminal compartment, a process providing a means of transport of spermatozoa into the epididymal ducts. Here, we describe a stopped-flow light-scattering methodology to easily assess the Sertoli cell osmotic water permeability. The devised methodology is well suited for studying how the Sertoli cell's permeability changes following exogenous stimuli. |
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