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B Cell Receptor Signaling

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Cover of 'B Cell Receptor Signaling'

Table of Contents

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    Book Overview
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    Chapter 1 Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution
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    Chapter 2 Analyzing Mouse B Cell Responses Specific to LCMV Infection
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    Chapter 3 Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors
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    Chapter 4 Biophysical Techniques to Study B Cell Activation: Single-Molecule Imaging and Force Measurements
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    Chapter 5 DNA-Based Probes for Measuring Mechanical Forces in Cell-Cell Contacts: Application to B Cell Antigen Extraction from Immune Synapses
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    Chapter 6 Deriving Quantitative Cell Biological Information from Dye-Dilution Lymphocyte Proliferation Experiments
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    Chapter 7 Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells
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    Chapter 8 Ex Vivo Culture Assay to Measure Human Follicular Helper T (Tfh) Cell-Mediated Human B Cell Proliferation and Differentiation
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    Chapter 9 B Cell Receptor Signaling and Compartmentalization by Confocal Microscopy
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    Chapter 10 Imaging the Interactions Between B Cells and Antigen-Presenting Cells
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    Chapter 11 In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes
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    Chapter 12 Study B Cell Antigen Receptor Nano-Scale Organization by In Situ Fab Proximity Ligation Assay
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    Chapter 13 Single-Particle Tracking of Cell Surface Proteins
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    Chapter 14 The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen
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    Chapter 15 Time-Lapse Förster Resonance Energy Transfer Imaging by Confocal Laser Scanning Microscopy for Analyzing Dynamic Molecular Interactions in the Plasma Membrane of B Cells
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    Chapter 16 Understanding of B Cell Receptor Signaling Through a Photo-Activatable Antigen Presentation System
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    Chapter 17 Use of Streptolysin O-Induced Membrane Damage as a Method of Studying the Function of Lipid Rafts During B Cell Activation
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    Chapter 18 Visualization and Quantitative Analysis of the Actin Cytoskeleton Upon B Cell Activation
Attention for Chapter 11: In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes
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Chapter title
In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes
Chapter number 11
Book title
B Cell Receptor Signaling
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7474-0_11
Pubmed ID
Book ISBNs
978-1-4939-7473-3, 978-1-4939-7474-0
Authors

Yolanda R. Carrasco

Abstract

The development of experimental systems that allow in vivo antigen tracking as well as the study of B cell dynamics in real time and in situ, have transformed our understanding of the "how, when and where" B lymphocytes find antigen at secondary lymphoid organs in the last 10 years. Here, I described one of these experimental models, which uses highly fluorescent particulate antigen and B cell receptor (BCR)-transgenic B cells labeled with long-term fluorescent probes, combined with confocal and multiphoton microscopy.

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Mendeley readers

The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 100%
Readers by discipline Count As %
Immunology and Microbiology 1 100%