| Chapter title |
Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells
|
|---|---|
| Chapter number | 7 |
| Book title |
B Cell Receptor Signaling
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7474-0_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7473-3, 978-1-4939-7474-0
|
| Authors |
Qizhao Huang, Haoqiang Wang, Lifan Xu, Jianjun Hu, Pengcheng Wang, Yiding Li, Lilin Ye |
| Abstract |
B lymphocytes and their differentiated daughter cells are charged with responding to invading pathogens and producing protective antibodies against these pathogens. The physiology of B cells is intimately connected with the function of the B cell antigen receptor (BCR). Upon activation of BCR, transmembrane signals are generated, and several downstream pathways are activated, which provide a primary directive for the cell's subsequent response. mTOR is a serine/threonine kinase that controls cell proliferation and metabolism in response to a diverse range of extracellular stimuli. The activation of mTOR signaling downstream of PI3K/Akt activity by B cell receptor (BCR) engagement has been generally assumed to be essential for B cell responses. This chapter seeks to present two protocols to evaluate mTOR activity in B cells bearing BCR specific to 4-hydroxy-3-nitrophenylacetyl (NP)-hapten. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 30% |
| Professor | 1 | 10% |
| Researcher | 1 | 10% |
| Student > Doctoral Student | 1 | 10% |
| Unknown | 4 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 4 | 40% |
| Chemistry | 1 | 10% |
| Medicine and Dentistry | 1 | 10% |
| Unknown | 4 | 40% |