| Chapter title |
Understanding of B Cell Receptor Signaling Through a Photo-Activatable Antigen Presentation System
|
|---|---|
| Chapter number | 16 |
| Book title |
B Cell Receptor Signaling
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7474-0_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7473-3, 978-1-4939-7474-0
|
| Authors |
Jing Wang, Zhengpeng Wan, Wanli Liu |
| Abstract |
Antibody responses are initiated by the binding of antigens to cell surface expressed B cell receptors (BCRs) that trigger signaling cascades resulting in the activation of B cells. However, it has been difficult to study these cascades due to their fast, dynamic, and transient nature. Using a conventional antigen-presenting system, such as planar lipid bilayers (PLBs), the initial events during B cell activation have been difficult to be captured. Here, we describe the general procedures for the utilization of a photoactivatable antigen-presenting system in the combination with a high speed live cell imaging method to investigate the early activation events in the same B cells in response to antigen stimulation. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 40% |
| Student > Postgraduate | 1 | 20% |
| Student > Bachelor | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Nursing and Health Professions | 1 | 20% |
| Psychology | 1 | 20% |
| Unknown | 2 | 40% |