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| Chapter title |
Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells
|
|---|---|
| Chapter number | 42 |
| Book title |
Sphingosine-1-Phosphate
|
| Published in |
Methods in molecular biology, May 2017
|
| DOI | 10.1007/7651_2017_42 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7412-2, 978-1-4939-7413-9
|
| Authors |
Alena P. Chumanevich, Piper A. Wedman, Carole A. Oskeritzian |
| Abstract |
Mast cells produce a potently bioactive sphingolipid metabolite sphingosine-1-phosphate (S1P) constitutively and upon activation. The ligation of S1P to its type 2 receptor on mast cells triggers a novel downstream signaling pathway that we discovered links activation of transcription factor signal transducer and activator of transcription 3 to mast cell-derived chemokine release in both humans and mice. In this chapter, we describe the methods used to study S1P signaling in human and mouse primary mast cells. |