Chapter title |
Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-parametric Flow Cytometry
|
---|---|
Chapter number | 24 |
Book title |
Sphingosine-1-Phosphate
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/7651_2017_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7412-2, 978-1-4939-7413-9
|
Authors |
Zhang, Jianhong, Bang, Annie, Lye, Stephen J., Jianhong Zhang, Annie Bang, Stephen J. Lye |
Abstract |
Flow cytometry is a powerful tool for phenotypic and functional analyses of single immune cells. The increasing capability of flow cytometry technology has driven a more detailed understanding of immune cell subsets and functions in complex cellular systems such as the developing human decidua/placenta. We propose a standardized procedure for the isolation and analysis of human decidual natural killer (dNK) cells and this method can be extended to investigation of other uterine lymphocytes. Here this platform is used to examine the expression of sphingosine-1-phosphate (S1P) receptor and functional growth factors by dNK cells. |
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