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Extracellular RNA

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Cover of 'Extracellular RNA'

Table of Contents

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    Book Overview
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    Chapter 1 Extracellular RNAs: A New Awareness of Old Perspectives
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    Chapter 2 Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles
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    Chapter 3 Extracellular RNA Isolation from Cell Culture Supernatant
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    Chapter 4 Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture
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    Chapter 5 Isolation of Extracellular RNA from Serum/Plasma
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    Chapter 6 Isolation of Extracellular RNA from Bile
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    Chapter 7 Cushioned–Density Gradient Ultracentrifugation (C-DGUC): A Refined and High Performance Method for the Isolation, Characterization, and Use of Exosomes
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    Chapter 8 Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids
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    Chapter 9 Enrichment of Extracellular Vesicle Subpopulations Via Affinity Chromatography
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    Chapter 10 Detection and Analysis of Non-vesicular Extracellular RNA
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    Chapter 11 Isolation of Plasma Lipoproteins as a Source of Extracellular RNA
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    Chapter 12 Droplet Digital PCR for Quantitation of Extracellular RNA
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    Chapter 13 Preparation of Small RNA NGS Libraries from Biofluids
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    Chapter 14 Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression Using NanoString
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    Chapter 15 Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs
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    Chapter 16 Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs
Attention for Chapter 12: Droplet Digital PCR for Quantitation of Extracellular RNA
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Chapter title
Droplet Digital PCR for Quantitation of Extracellular RNA
Chapter number 12
Book title
Extracellular RNA
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7652-2_12
Pubmed ID
Book ISBNs
978-1-4939-7651-5, 978-1-4939-7652-2
Authors

Irene K. Yan, Rishabh Lohray, Tushar Patel

Abstract

Cell-to-cell communication involves the release of biological molecules into the extracellular space and their uptake by recipient cells. These molecules include RNA that can modulate cellular signaling and biological processes. To study extracellular RNA, highly sensitive and precise methods for their detection are needed. Digital polymerase chain reaction (dPCR) can be a useful method for detecting and analyzing extracellular RNA. The sensitivity of digital PCR can exceed that of quantitative PCR for low abundance targets such as extracellular RNA.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Other 3 27%
Professor 1 9%
Student > Ph. D. Student 1 9%
Researcher 1 9%
Professor > Associate Professor 1 9%
Other 1 9%
Unknown 3 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 27%
Medicine and Dentistry 2 18%
Immunology and Microbiology 1 9%
Agricultural and Biological Sciences 1 9%
Unknown 4 36%