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Extracellular RNA

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Cover of 'Extracellular RNA'

Table of Contents

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    Book Overview
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    Chapter 1 Extracellular RNAs: A New Awareness of Old Perspectives
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    Chapter 2 Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles
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    Chapter 3 Extracellular RNA Isolation from Cell Culture Supernatant
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    Chapter 4 Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture
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    Chapter 5 Isolation of Extracellular RNA from Serum/Plasma
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    Chapter 6 Isolation of Extracellular RNA from Bile
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    Chapter 7 Cushioned–Density Gradient Ultracentrifugation (C-DGUC): A Refined and High Performance Method for the Isolation, Characterization, and Use of Exosomes
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    Chapter 8 Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids
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    Chapter 9 Enrichment of Extracellular Vesicle Subpopulations Via Affinity Chromatography
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    Chapter 10 Detection and Analysis of Non-vesicular Extracellular RNA
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    Chapter 11 Isolation of Plasma Lipoproteins as a Source of Extracellular RNA
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    Chapter 12 Droplet Digital PCR for Quantitation of Extracellular RNA
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    Chapter 13 Preparation of Small RNA NGS Libraries from Biofluids
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    Chapter 14 Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression Using NanoString
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    Chapter 15 Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs
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    Chapter 16 Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs
Attention for Chapter 5: Isolation of Extracellular RNA from Serum/Plasma
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Chapter title
Isolation of Extracellular RNA from Serum/Plasma
Chapter number 5
Book title
Extracellular RNA
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7652-2_5
Pubmed ID
Book ISBNs
978-1-4939-7651-5, 978-1-4939-7652-2
Authors

Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang, Leonora Balaj, Saumya Das, Roopali Gandhi, Louise C. Laurent, Anil K. Sood

Abstract

Extracellular RNAs are initiating increased interest due to their potentials in serving as novel biomarkers, mediators of intercellular communication, and therapeutic applications. As a newly emerging field, one of the main obstacles is the lack of standardized protocols for RNA isolations. Here we describe protocols for commercially available kits that have been modified to yield consistent results for isolation of extracellular RNA from both whole serum/plasma and extracellular vesicle-enriched serum/plasma samples.

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Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 23%
Student > Master 4 13%
Student > Bachelor 3 10%
Student > Ph. D. Student 2 7%
Librarian 1 3%
Other 2 7%
Unknown 11 37%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 23%
Agricultural and Biological Sciences 4 13%
Environmental Science 1 3%
Chemical Engineering 1 3%
Immunology and Microbiology 1 3%
Other 3 10%
Unknown 13 43%