Extracellular RNA
Humana Press, New York, NY
Chapter title |
Extracellular RNA Isolation from Cell Culture Supernatant
|
---|---|
Chapter number | 3 |
Book title |
Extracellular RNA
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7652-2_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7651-5, 978-1-4939-7652-2
|
Authors |
Sawen Bakr, Bridget Simonson, Kirsty M. Danielson, Saumya Das |
Abstract |
Extracellular RNAs are emerging as novel biomarkers and mediators of intercellular communication. Various methods to isolate RNA from biofluids and cell culture supernatants have been previously used by investigators. Here, we describe several standardized protocols for the isolation of RNAs from cell culture supernatants that utilize commercially available kits and reagents. |
Country | Count | As % |
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Unknown | 19 | 100% |
Readers by professional status | Count | As % |
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Researcher | 4 | 21% |
Student > Ph. D. Student | 3 | 16% |
Student > Bachelor | 3 | 16% |
Student > Doctoral Student | 2 | 11% |
Student > Master | 1 | 5% |
Other | 1 | 5% |
Unknown | 5 | 26% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 7 | 37% |
Medicine and Dentistry | 2 | 11% |
Chemical Engineering | 1 | 5% |
Agricultural and Biological Sciences | 1 | 5% |
Business, Management and Accounting | 1 | 5% |
Other | 2 | 11% |
Unknown | 5 | 26% |