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Mendeley readers
| Chapter title |
Nonradioactive Northern Blot of circRNAs
|
|---|---|
| Chapter number | 11 |
| Book title |
Circular RNAs
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7562-4_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7561-7, 978-1-4939-7562-4
|
| Authors |
Xiaolin Wang, Ge Shan |
| Abstract |
Circular RNAs (circRNAs) are recognized as a special species of transcripts in metazoans with increasing studies, and northern blotting is a direct way to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (32P) labeled, which is not environment-friendly and sometimes inconvenient to use. Here, we describe a nonradioactive northern blot protocol with digoxigenin-labeled probe to detect circRNA. |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 21% |
| Student > Ph. D. Student | 4 | 21% |
| Student > Bachelor | 3 | 16% |
| Researcher | 2 | 11% |
| Student > Doctoral Student | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 4 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 32% |
| Neuroscience | 4 | 21% |
| Immunology and Microbiology | 2 | 11% |
| Agricultural and Biological Sciences | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 4 | 21% |