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Mendeley readers
| Chapter title |
Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization
|
|---|---|
| Chapter number | 6 |
| Book title |
Circular RNAs
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7562-4_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7561-7, 978-1-4939-7562-4
|
| Authors |
Anne Zirkel, Argyris Papantonis |
| Abstract |
Fluorescence in situ hybridization (FISH) coupled to high-resolution microscopy is a powerful method for analyzing the subcellular localization of RNA. However, the detection of circular RNAs (circRNAs) using microscopy is challenging because the only feature of a circRNA that can be used for the probe design is its junction. Circular RNAs are expressed at varying levels, and for their efficient monitoring by FISH, background fluorescence levels need to be kept low. Here, we describe a FISH protocol coupled to high-precision localizations using a single fluorescently labeled probe spanning the circRNA junction; this allows circRNA detection in mammalian cells with high signal-to-noise ratios. |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 8 | 32% |
| Student > Ph. D. Student | 5 | 20% |
| Student > Master | 3 | 12% |
| Professor > Associate Professor | 2 | 8% |
| Researcher | 2 | 8% |
| Other | 1 | 4% |
| Unknown | 4 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 44% |
| Agricultural and Biological Sciences | 3 | 12% |
| Chemistry | 2 | 8% |
| Neuroscience | 2 | 8% |
| Environmental Science | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 4 | 16% |