You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
An Assay for Detecting RNA Topoisomerase Activity
|
---|---|
Chapter number | 12 |
Book title |
DNA Topoisomerases
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7459-7_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7458-0, 978-1-4939-7459-7
|
Authors |
Muzammil Ahmad, Dongyi Xu, Weidong Wang |
Abstract |
RNA topoisomerase activity has recently been detected in multiple Type IA DNA topoisomerases from all three domains of life: bacteria, archaea, and eukarya. Many, but not all, Type IA topoisomerases are found to possess activities for not only DNA, but also RNA, suggesting that they may solve topological problems for both types of nucleic acids. Here we describe a detailed assay used by our group to detect RNA topoisomerase activity for many Type IA topoisomerases. We discuss the strategy, experimental procedures, troubleshooting, and limitations for this assay. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 50% |
Researcher | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 50% |
Neuroscience | 1 | 50% |