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Mendeley readers
Chapter title |
DNA Catenation Reveals the Dynamics of DNA Topology During Replication
|
---|---|
Chapter number | 5 |
Book title |
DNA Topoisomerases
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7459-7_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7458-0, 978-1-4939-7459-7
|
Authors |
Alicia Castán, Pablo Hernández, Dora B. Krimer, Jorge B. Schvartzman |
Abstract |
Two-dimensional agarose gel electrophoresis is the method of choice to identify and quantify all the topological forms DNA molecules can adopt in vivo. Here we describe the materials and protocols needed to analyze catenanes, the natural outcome of DNA replication, in Saccharomyces cerevisiae. We describe the formation of pre-catenanes during replication and how inhibition of topoisomerase 2 leads to the accumulation of intertwined sister duplexes. This knowledge is essential to determine how replication forks blockage or pausing affects the dynamic of DNA topology during replication. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 33% |
Student > Bachelor | 2 | 22% |
Librarian | 1 | 11% |
Student > Ph. D. Student | 1 | 11% |
Unknown | 2 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 33% |
Agricultural and Biological Sciences | 3 | 33% |
Engineering | 1 | 11% |
Unknown | 2 | 22% |