Chapter title |
A Practical Guide to iSPOT Modeling: An Integrative Structural Biology Platform
|
---|---|
Chapter number | 14 |
Book title |
Biological Small Angle Scattering: Techniques, Strategies and Tips
|
Published in |
Advances in experimental medicine and biology, January 2017
|
DOI | 10.1007/978-981-10-6038-0_14 |
Pubmed ID | |
Book ISBNs |
978-9-81-106037-3, 978-9-81-106038-0
|
Authors |
An Hsieh, Lanyuan Lu, Mark R. Chance, Sichun Yang |
Abstract |
Integrative structure modeling is an emerging method for structural determination of protein-protein complexes that are challenging for conventional structural techniques. Here, we provide a practical protocol for implementing our integrated iSPOT platform by integrating three different biophysical techniques: small-angle X-ray scattering (SAXS), hydroxyl radical footprinting, and computational docking simulations. Specifically, individual techniques are described from experimental and/or computational perspectives, and complementary structural information from these different techniques are integrated for accurate characterization of the structures of large protein-protein complexes. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 25% |
Student > Bachelor | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Student > Master | 1 | 13% |
Student > Ph. D. Student | 1 | 13% |
Other | 0 | 0% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 25% |
Chemistry | 2 | 25% |
Agricultural and Biological Sciences | 1 | 13% |
Unknown | 3 | 38% |