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Biological Small Angle Scattering: Techniques, Strategies and Tips

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Cover of 'Biological Small Angle Scattering: Techniques, Strategies and Tips'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Small Angle Scattering: Historical Perspective and Future Outlook
  3. Altmetric Badge
    Chapter 2 Sample and Buffer Preparation for SAXS
  4. Altmetric Badge
    Chapter 3 Considerations for Sample Preparation Using Size-Exclusion Chromatography for Home and Synchrotron Sources
  5. Altmetric Badge
    Chapter 4 How to Analyze and Present SAS Data for Publication
  6. Altmetric Badge
    Chapter 5 Designing and Performing Biological Solution Small-Angle Neutron Scattering Contrast Variation Experiments on Multi-component Assemblies
  7. Altmetric Badge
    Chapter 6 SAS-Based Structural Modelling and Model Validation
  8. Altmetric Badge
    Chapter 7 Structural Characterization of Highly Flexible Proteins by Small-Angle Scattering
  9. Altmetric Badge
    Chapter 8 What Can We Learn from Wide-Angle Solution Scattering?
  10. Altmetric Badge
    Chapter 9 SAS-Based Studies of Protein Fibrillation
  11. Altmetric Badge
    Chapter 10 High Resolution Distance Distributions Determined by X-Ray and Neutron Scattering
  12. Altmetric Badge
    Chapter 11 A Successful Combination: Coupling SE-HPLC with SAXS
  13. Altmetric Badge
    Chapter 12 Applications of SANS to Study Membrane Protein Systems
  14. Altmetric Badge
    Chapter 13 Hybrid Applications of Solution Scattering to Aid Structural Biology
  15. Altmetric Badge
    Chapter 14 A Practical Guide to iSPOT Modeling: An Integrative Structural Biology Platform
  16. Altmetric Badge
    Chapter 15 Small Angle Scattering for Pharmaceutical Applications: From Drugs to Drug Delivery Systems
Attention for Chapter 14: A Practical Guide to iSPOT Modeling: An Integrative Structural Biology Platform
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Chapter title
A Practical Guide to iSPOT Modeling: An Integrative Structural Biology Platform
Chapter number 14
Book title
Biological Small Angle Scattering: Techniques, Strategies and Tips
Published in
Advances in experimental medicine and biology, January 2017
DOI 10.1007/978-981-10-6038-0_14
Pubmed ID
Book ISBNs
978-9-81-106037-3, 978-9-81-106038-0
Authors

An Hsieh, Lanyuan Lu, Mark R. Chance, Sichun Yang

Abstract

Integrative structure modeling is an emerging method for structural determination of protein-protein complexes that are challenging for conventional structural techniques. Here, we provide a practical protocol for implementing our integrated iSPOT platform by integrating three different biophysical techniques: small-angle X-ray scattering (SAXS), hydroxyl radical footprinting, and computational docking simulations. Specifically, individual techniques are described from experimental and/or computational perspectives, and complementary structural information from these different techniques are integrated for accurate characterization of the structures of large protein-protein complexes.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 25%
Student > Bachelor 1 13%
Student > Doctoral Student 1 13%
Student > Master 1 13%
Student > Ph. D. Student 1 13%
Other 0 0%
Unknown 2 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 25%
Chemistry 2 25%
Agricultural and Biological Sciences 1 13%
Unknown 3 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 December 2017.
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#18,578,649
of 23,011,300 outputs
Outputs from Advances in experimental medicine and biology
#3,325
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Outputs of similar age
#311,468
of 421,287 outputs
Outputs of similar age from Advances in experimental medicine and biology
#333
of 490 outputs
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So far Altmetric has tracked 4,960 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one is in the 19th percentile – i.e., 19% of its peers scored the same or lower than it.
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