| Chapter title |
Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-State NMR of Microcrystalline and Membrane-Bound Proteins
|
|---|---|
| Chapter number | 2 |
| Book title |
Protein NMR
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7386-6_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7385-9, 978-1-4939-7386-6
|
| Authors |
Gopinath, T, Veglia, Gianluigi, T. Gopinath, Gianluigi Veglia |
| Abstract |
Conventional NMR pulse sequences record one spectrum per experiment, while spending most of the time waiting for the spin system to return to the equilibrium. As a result, a full set of multidimensional NMR experiments for biological macromolecules may take up to several months to complete. Here, we present a practical guide for setting up a new class of MAS solid-state NMR experiments (POE or polarization optimized experiments) that enable the simultaneous acquisition of multiple spectra of proteins, accelerating data acquisition. POE exploit the long-lived (15)N polarization of isotopically labeled proteins and enable one to obtain up to eight spectra, by concatenating classical NMR pulse sequences. This new strategy propels data throughput of solid-state NMR spectroscopy of fibers, microcrystalline preparations, as well as membrane proteins. |
X Demographics
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| Japan | 1 | 100% |
Demographic breakdown
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|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
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| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 29% |
| Other | 2 | 29% |
| Student > Bachelor | 1 | 14% |
| Professor | 1 | 14% |
| Unknown | 1 | 14% |
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| Unknown | 2 | 29% |