| Chapter title |
Quantitative Determination of Interacting Protein Surfaces in Prokaryotes and Eukaryotes by Using In-Cell NMR Spectroscopy
|
|---|---|
| Chapter number | 20 |
| Book title |
Protein NMR
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7386-6_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7385-9, 978-1-4939-7386-6
|
| Authors |
David S. Burz, Christopher M. DeMott, Asma Aldousary, Stephen Dansereau, Alexander Shekhtman |
| Abstract |
This paper describes three protocols for identifying interacting surfaces on (15)N-labeled target proteins of known structure by using in-cell NMR spectroscopy. The first protocol describes how to identify protein quinary structure interaction surfaces in prokaryotes by using cross-relaxation-induced polarization transfer, CRIPT, based in-cell NMR. The second protocol describes how to introduce labeled protein into eukaryotic (HeLa) cells via electroporation for CRIPT-based in-cell studies. The third protocol describes how to quantitatively map protein interacting surfaces by utilizing singular value decomposition, SVD, analysis of STructural INTeractions by in-cell NMR, STINT-NMR, data. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 25% |
| Student > Ph. D. Student | 3 | 25% |
| Researcher | 2 | 17% |
| Professor | 1 | 8% |
| Student > Postgraduate | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 2 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 4 | 33% |
| Biochemistry, Genetics and Molecular Biology | 3 | 25% |
| Computer Science | 1 | 8% |
| Agricultural and Biological Sciences | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 1 | 8% |