Chapter title |
Structures of Dynamic Protein Complexes: Hybrid Techniques to Study MAP Kinase Complexes and the ESCRT System
|
---|---|
Chapter number | 17 |
Book title |
Protein NMR
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7386-6_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7385-9, 978-1-4939-7386-6
|
Authors |
Wolfgang Peti, Rebecca Page, Evzen Boura, Bartosz Różycki |
Abstract |
The integration of complementary molecular methods (including X-ray crystallography, NMR spectroscopy, small angle X-ray/neutron scattering, and computational techniques) is frequently required to obtain a comprehensive understanding of dynamic macromolecular complexes. In particular, these techniques are critical for studying intrinsically disordered protein regions (IDRs) or intrinsically disordered proteins (IDPs) that are part of large protein:protein complexes. Here, we explain how to prepare IDP samples suitable for study using NMR spectroscopy, and describe a novel SAXS modeling method (ensemble refinement of SAXS; EROS) that integrates the results from complementary methods, including crystal structures and NMR chemical shift perturbations, among others, to accurately model SAXS data and describe ensemble structures of dynamic macromolecular complexes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 30% |
Professor | 2 | 20% |
Student > Bachelor | 2 | 20% |
Student > Ph. D. Student | 2 | 20% |
Student > Master | 1 | 10% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 40% |
Physics and Astronomy | 3 | 30% |
Chemistry | 2 | 20% |
Agricultural and Biological Sciences | 1 | 10% |